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High-sensitive and rapid detection of Mycobacterium tuberculosis infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area(Open Access)

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资源类型：
Pubmed体系：
作者：
Jiang, W[a] * ; Shao, L[a] ; Zhang, Y[b] ; Zhang, S[a] ; Meng, C[a] ; Xu, Y[c] ; Huang, L[a] ; Wang, Y[a] ; Wang, Y[a] ; Weng, X[a] ; Zhang, W[a] ;
机构： [a]Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China [b]Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, United States [c]Department of Infectious Diseases, Honghe No.1 People's Hospital, Yunnan Province 661100, China
出处：
DOI： 10.1186/1471-2172-10-31
ISSN：

摘要：
Background: An accurate test for Mycobacterium tuberculosis infection is urgently needed in immunosuppressed populations. The aim of this study was to investigate the diagnostic power of enzyme-linked immunospot (ELISPOT)-based IFN-γ release assay in detecting active and latent tuberculosis in HIV-infected population in bacillus Calmette-Guerin (BCG)-vaccinated area. A total of 100 HIV-infected individuals including 32 active tuberculosis patients were recruited. An ELISPOT-based IFN-γ release assay, T-SPOT.TB, was used to evaluate the M. tuberculosis ESAT-6 and CFP-10 specific IFN-γ response. Tuberculin skin test (TST) was performed for all recruited subjects. Results: The subjects were divided into group HIV+ATB (HIV-infected individuals with active tuberculosis, n = 32), group HIV+LTB (HIV-infected individuals with positive results of T-SPOT.TB assay, n = 46) and group HIV only (HIV-infected individuals with negative results of T-SPOT.TB assay and without evidence of tuberculosis infection, n = 22). In group HIV+ATB and HIV+LTB, T-SPOT.TB positive rate in subjects with TST <5 mm were 50% (16/32) and 41.3% (19/46), respectively. Individuals in group HIV+ATB and HIV+LTB with CD4+ T cells <500/ μl, T-SPOT.TB showed a higher sensitivity than TST (64.5% vs. 22.6% and 62.2% vs. 29.7%, respectively, both P < 0.0001). In addition, the sensitivity of T-SPOT.TB assay in group HIV+ATB increased to >85% in patients with TB treatment for less than 1 month and CD4+ T cells ≥200/μl, while for patients treated for more than 3 months and CD4+ T cells <200/μl, the sensitivity was decreased to only 33.3%. Furthermore, the results could be generated by T-SPOT.TB assay within 24 hours, which was more rapid than TST with 48-72 hours. Conclusion: ELISPOT-based IFN-γ release assay is more sensitive and rapid for the diagnosis of TB infection in Chinese HIV-infected individuals with history of BCG vaccination, and could be an effective tool for guiding preventive treatment with isoniazid in latently infected people and for TB control in China. © 2009 Jiang et al; licensee BioMed Central Ltd.

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外文
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中科院(CAS)分区：
出版当年[2009]版：
大类 | 3 区 医学
小类 | 3 区 免疫学
最新[2023]版：
大类 | 4 区 医学
小类 | 4 区 免疫学
第一作者：
第一作者机构： [a]Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China
通讯作者：
推荐引用方式(GB/T 7714)：
Jiang W,Shao L,Zhang Y,et al.High-sensitive and rapid detection of Mycobacterium tuberculosis infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area(Open Access)[J].BMC immunology.2009,10:31.doi:10.1186/1471-2172-10-31.
APA：
Jiang, W,Shao, L,Zhang, Y,Zhang, S,Meng, C...&Zhang, W.(2009).High-sensitive and rapid detection of Mycobacterium tuberculosis infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area(Open Access).BMC immunology,10,
MLA：
Jiang, W,et al."High-sensitive and rapid detection of Mycobacterium tuberculosis infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area(Open Access)".BMC immunology 10.(2009):31

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