机构：[1]College of Clinical Medicine, Dali University, Dali 671000, Yunnan Province, China.[2]Department of Ophthalmology, Fuling Central Hospital of Chongqing City, Fuling 408000, Chongqing Province, China.[3]Department of Ophthalmology, the First Affiliated Hospital of Dali University, Dali 671000, Yunnan Province, China.[4]Department of Endocrinology, the First Affiliated Hospital of Dali University, Dali 671000, Yunnan Province, China.
To investigate the anti-proliferation and apoptosis-inducing effects of sodium aescinate (SA) on retinoblastoma Y79 cells and its mechanism.Y79 cells were cultured at different drug concentrations for different periods of time (24, 48, and 72h). The inhibitory effect of SA on proliferation of Y79 cells was detected by the cell counting kit-8 (CCK-8) assay, and the morphology of Y79 cells in each group was observed under an inverted microscope. An IC50 of 48h was selected for subsequent experiments. After pretreatment with SA for 24 and 48h, cellular DNA distribution and apoptosis were detected by flow cytometry. Real-time qunatitative polymerase chain reaction (RT-qPCR) and Western blot were used to assess changes in related genes (CDK1, CyclinB1, Bax, Bcl-2, caspase-9, caspase-8, and caspase-3).SA inhibited proliferation and induced apoptosis of Y79 cells in a time-dependent and concentration-dependent manner. Following its intervention in the cell cycle pathway, SA can inhibit the expression of CDK1 and CyclinB1 at the mRNA and protein levels, and block cells in the G2/M phase. In caspase-related apoptotic pathways, up-regulation of Bax and down-regulation of Bcl-2 caused caspase-9 to self-cleave and further activate caspase-3. What's more, the caspase-8-mediated extrinsic apoptosis pathway was activated, and the activated caspase-8 was released into the cytoplasm to activate caspase-3, which as a member of the downstream apoptotic effect group, initiates a caspase-cascade reaction that induces cell apoptosis.SA inhibits the proliferation of Y79 cells by arresting the cell cycle at the G2/M phase, and induces apoptosis via the caspase-related apoptosis pathway, indicating that SA may have promising potential as a chemotherapeutic drug.International Journal of Ophthalmology Press.
基金：
Supported by the National Natural Science
Foundation of China (No.81260153); Scientific Research
Fund Project of Yunnan Education Department, China
(N〇.2019Y0278).
第一作者机构：[1]College of Clinical Medicine, Dali University, Dali 671000, Yunnan Province, China.
共同第一作者：
通讯作者：
通讯机构：[3]Department of Ophthalmology, the First Affiliated Hospital of Dali University, Dali 671000, Yunnan Province, China.[4]Department of Endocrinology, the First Affiliated Hospital of Dali University, Dali 671000, Yunnan Province, China.[*1]Department of Ophthalmology, the First Affiliated Hospital of Dali University, Dali 671000, Yunnan Province, China,[*2]Department of Endocrinology, the First Affiliated Hospital of Dali University, Dali 671000, Yunnan Province, China.
推荐引用方式(GB/T 7714)：
Li Lei,Xu Bing,Li Cai-Rui,et al.Anti-proliferation and apoptosis-inducing effects of sodium aescinate on retinoblastoma Y79 cells[J].INTERNATIONAL JOURNAL OF OPHTHALMOLOGY.2020,13(10):1546-1553.doi:10.18240/ijo.2020.10.06.
APA：
Li Lei,Xu Bing,Li Cai-Rui,Zhang Miao-Miao,Wu Sheng-Jun...&Zhao Wei.(2020).Anti-proliferation and apoptosis-inducing effects of sodium aescinate on retinoblastoma Y79 cells.INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,13,(10)
MLA：
Li Lei,et al."Anti-proliferation and apoptosis-inducing effects of sodium aescinate on retinoblastoma Y79 cells".INTERNATIONAL JOURNAL OF OPHTHALMOLOGY 13..10(2020):1546-1553
