机构：[1]Department of Pathology, the Second Hospital of Tianjin Medical University, Tianjin[2]Yunnan University of[3]Department of Pathology, The First People’s Hospital of Yunnan Province,医技片病理科云南省第一人民医院[4]Department of General Surgery, the Second Hospital of Tianjin Medical University, Tianjin, China
Objective. This study aimed to investigate the effects of combined activin A and Wnt3a treatment on definitive endoderm (DE) differentiation from human parthenogenetic embryonic stem cells (hPESCs). Methods. hPESCs on human foreskin fibroblast feeder layers were induced to differentiate into DE using a combination of 50 ng/ml activin A and 25 ng/ml Wnt3a. Expression of the DE markers CXCR4, E-cadherin (ECD), Sox17, and Goosecoid (Gsc) were examined using flow cytometry and real-time quantitative PCR. Results. The combination of activin A and Wnt3a significantly enhanced the percentages of CXCR4(+), ECD+, Sox17(+), and Gsc(+) cells, culminating on day 2 of induction. This combined use promoted DE differentiation from hPESCs in vitro. Conclusion. Through the combination treatment using activin A and Wnt3a, DE differentiation from hPESCs culminated at 48 h, which can be regarded as the optimal time-point to induce differentiation of endodermal cells such as pancreatic, liver, and intestinal cells.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81660302]; Applied Basic Research Foundation of Yunnan Province [2013FZ179, 2014FB040]; Youth Foundation of the second hospital of Tianjin Medical University [2019ydey02, 2019ydey09]
第一作者机构：[1]Department of Pathology, the Second Hospital of Tianjin Medical University, Tianjin
共同第一作者：
通讯作者：
通讯机构：[4]Department of General Surgery, the Second Hospital of Tianjin Medical University, Tianjin, China[*1]Department of General Surgery, The Second Hospital of Tianjin Medical University, No. 23 Pingjiang Road Hexi District, Tianjin 300211, China
推荐引用方式(GB/T 7714)：
Liang Rui,Xiao Xiaoxiao,Luo Lilin,et al.Efficient Definitive Endoderm Differentiation from Human Parthenogenetic Embryonic Stem Cells Induced by Activin A and Wnt3a[J].ANNALS OF CLINICAL AND LABORATORY SCIENCE.2020,50(4):468-473.
APA：
Liang, Rui,Xiao, Xiaoxiao,Luo, Lilin,Chen, Tianxing,Yang, Hui...&Wang, Zhiqiang.(2020).Efficient Definitive Endoderm Differentiation from Human Parthenogenetic Embryonic Stem Cells Induced by Activin A and Wnt3a.ANNALS OF CLINICAL AND LABORATORY SCIENCE,50,(4)
MLA：
Liang, Rui,et al."Efficient Definitive Endoderm Differentiation from Human Parthenogenetic Embryonic Stem Cells Induced by Activin A and Wnt3a".ANNALS OF CLINICAL AND LABORATORY SCIENCE 50..4(2020):468-473
