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An Effective Acid-Base-Induced Liquid-Liquid Microextraction Based on Deep Eutectic Solvents for Determination of Testosterone and Methyltestosterone in Milk

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机构： [1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China, [2]Institute of Basic and Clinical Medicine, Provincial Key Laboratory for Virology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China, [3]Department of Gynaecology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China

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An environmentally friendly method for the determination of testosterone and methyltestosterone by acid-base-induced deep eutectic solvents liquid-liquid microextraction (DES-ABLLME) combining with high-performance liquid chromatography was established. The deep eutectic solvent (DES) consisting of menthol:lauric acid:decanoic acid (3:1:1) can act as both hydrogen bond donor and hydrogen bond acceptor. In this approach, ammonia solution (NH3•H2O) is used as an emulsifier to react with DESs in the extraction process to generate salt and form milky white solution, achieving high extraction efficiency. Hydrochloric acid was used as a phase separator to change the emulsification state and promote the separation of extraction agent from water phase. A series of parameters were optimized including the volume of DES and the emulsifying agent, glucose concentration as well as hydrochloric acid volume. The method was linear in the range 0.5-100 μg mL-1 with a correlation coefficient (R) of 0.9999, and the limits of detection were 0.067 and 0.2 μg mL-1 for testosterone and methyltestosterone, respectively. This method was applied to analyze testosterone and methyltestosterone in milk samples, and the recoveries were between 89.2 and 108.2%. © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: [email protected]

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出版当年[2020]版：

大类 | 4 区化学

小类 | 4 区生化研究方法 4 区分析化学

最新[2025]版：

大类 | 4 区化学

小类 | 4 区生化研究方法 4 区分析化学

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出版当年[2019]版：

Q4 BIOCHEMICAL RESEARCH METHODS Q4 CHEMISTRY, ANALYTICAL

最新[2023]版：

Q3 CHEMISTRY, ANALYTICAL Q4 BIOCHEMICAL RESEARCH METHODS

影响因子： 1.5 最新[2023版] 1.6 最新五年平均 1.28 出版当年[2019版] 1.28 出版当年五年平均 1.216 出版前一年[2018版] 1.618 出版后一年[2020版]

第一作者：

第一作者机构： [1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China, [2]Institute of Basic and Clinical Medicine, Provincial Key Laboratory for Virology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China, [3]Department of Gynaecology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China

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