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Synergistic effect of chlorogenic acid and levofloxacin against Klebsiella pneumonia infection in vitro and in vivo(Open Access)

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机构: [1]School of Agriculture, Chenggong Campus, Yunnan University, South Section, East Outer Ring Road, Chenggong District, Kunming 650500, People’s Republic of China [2]Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming 650500, People’s Republic of China [3]Dehong Tropical Agriculture Research Institute of Yunnan, Ruili 678600, People’s Republic of China [4]School of Life Sciences, Yunnan Normal University, No.1, Yuhua Area, Chenggong District, Kunming 650500, Yunnan, People’s Republic of China [5]The Second Afliated Hospital of Kunming Medical University, Kunming 650101, People’s Republic of China [6]Department of Paediatrics, The First People’s Hospital of Yunnan Province, The Afliated Hospital of Kunming University of Science and Technology, 157 Jinbi Road, Kunming 650032, People’s Republic of China [7]Yunnan Clinical Medical Center for Hematological Diseases, The First People’s Hospital of Yunnan Province, 157 Jinbi Road, Kunming 650032, People’s Republic of China
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The study aimed to investigate the antibacterial effect and potential mechanisms of chlorogenic acid (CA) in Klebsiella pneumonia (KPN) induced infection in vitro and in vivo. 62 KPN strains were collected from the First People’s Hospital of Yunnan Province. CA and CA combined Levofloxacin (LFX) were detected for KPN biofilm (BF) formation in vitro. The lung infection mice model were established by KPN. The effect of CA (500 mg/kg), LFX (50 mg/kg) and CA combined LFX (250 mg/kg + 25 mg/kg) were evaluated through the survival of mice, the changes of inflammation factors of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β and IL-6 in serum, the histopathological analysis of lung and the protein expression of NLRP3 signaling pathway in vivo. A total of 62 KPNs were isolated and identified, of which 13 (21%) strains were BF positive. 8 (13%) strains were extended spectrum β-lactamase strains (ESBLs), and 20 (32%) strains are ESBLs biofilm positive. In vitro study, CA and LFX showed a synergistic effect on KPN biofilm formation. In vivo mice experiment, CA, especially CA + LFX treated group significantly decreased the serum levels of TNF-α, IL-1β and IL-6, improved the survival ratio and lung pathology changes, and also reduced the protein expression of ASC, caspase 1 p20, IL-1β and phosphor NF-κB p65. CA could effectively alleviate lung infection of KPN infected mice, and the antibacterial effection is strengthened by combined with LFX. The study provide a theroy basis for making rational and scientific antibacterial therapy strategy in clinic. © 2020, The Author(s).

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出版当年[2020]版:
大类 | 3 区 综合性期刊
小类 | 3 区 综合性期刊
最新[2023]版:
大类 | 2 区 综合性期刊
小类 | 2 区 综合性期刊
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出版当年[2019]版:
Q1 MULTIDISCIPLINARY SCIENCES
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Q1 MULTIDISCIPLINARY SCIENCES

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第一作者机构: [1]School of Agriculture, Chenggong Campus, Yunnan University, South Section, East Outer Ring Road, Chenggong District, Kunming 650500, People’s Republic of China [2]Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming 650500, People’s Republic of China
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通讯机构: [6]Department of Paediatrics, The First People’s Hospital of Yunnan Province, The Afliated Hospital of Kunming University of Science and Technology, 157 Jinbi Road, Kunming 650032, People’s Republic of China [7]Yunnan Clinical Medical Center for Hematological Diseases, The First People’s Hospital of Yunnan Province, 157 Jinbi Road, Kunming 650032, People’s Republic of China
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