机构：[1]Yunnan Provincial, Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Yunnan, China. Acquisition of data, manuscript preparation.[2]National-Local Joint Engineering Research Center of Entomoceutics, Dali University, Yunnan, China. Critical revision, final approval.[3]Master, The People’s Hospital of Linqing, Shandong Province, China. Acquisition, analysis and interpretation of data.[4]Yunnan Provincial, Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Yunnan, China. Conception and design of the study, manuscript writing, final approval.[5]The First People’s Hospital of Yunnan Province, Kunming, Yunnan Province, China. Histopathological examinations, critical revision, final approval.医技片病理科云南省第一人民医院
To investigate the mechanism of Periplaneta americana extract promoting intestinal mucosal repair of OXZ-induced colitis in rat.
All experiments used an equal number of male and female SD rats (n=48). We injected OXZ into the colon to induce UC rat model. To determine the optimal concentration of P. Americana's extract (PA-40), it was classified into low (L), medium (M), and high (H) doses. After OXZ treatment, each drug was administered by enema for 7 consecutive days. Rats were divided into the following 6 groups: (1) Saline treatment group (NC), (2) OXZ treatment UC model group (MC), (3) OXZ + budesonide group (BUN), (4) OXZ + PA-40 L group, (5) OXZ + PA-40 M group, (6) OXZ + PA-40 H group. Disease activity index (DAI) scores, colon length, histopathological score, serum cytokine level (IL-4, IL-10, iNOS, tNOS), and amount of MPO, EGF, IL-13 in colonic mucosa were measured.
PA treatment had a significant healing effect on the OXZ-colitis model and significantly reduced the lesioned area, especially in the PA-40H groups. PA treatment did not alter the expression of IL-10 and MPO level, but increased EGF (epidermal growth factor) and decrease IL-13 in the colonic tissue. PA inhibited the rise of NOSs (nitric oxide synthase) and decreased the serum IL-4 level.
The data suggest that Periplaneta americana extract may be a potential compound for the treatment of colonic lesions. The mechanism may be related to inhibiting the secretion of IL-13 and promoting the formation of EGF.
基金：
The National Natural Science Fund of
China (NO.81660186, 81660605, 81860742, 81860765);
Yunnan Fundamental Research Projects (grant
NO. 2017FH001-108, 2018FB143, 2019FH001-003,
202001AT070021); Innovation Team of Dali University
for Advanced Pharmaceutics of Entomological Biopharmacy
R&D (NO.ZKLX2019101); Innovative Team
of Dali University for Digital Development of Medicinal
Insects and Arachnids Resources (NO.ZKLX2020303);
Special Fund for Traditional Chinese Medicine Pieces of
Department of Industry and Information Technology of
Yunnan Province (NO.2019-YG-067)
第一作者机构：[1]Yunnan Provincial, Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Yunnan, China. Acquisition of data, manuscript preparation.
共同第一作者：
通讯作者：
通讯机构：[2]National-Local Joint Engineering Research Center of Entomoceutics, Dali University, Yunnan, China. Critical revision, final approval.[*1]Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D Dali, Yunnan China
推荐引用方式(GB/T 7714)：
Xue Nan-Nan,He Miao,Li Yue,et al.Periplaneta americana extract promotes intestinal mucosa repair of ulcerative colitis in rat.[J].ACTA CIRURGICA BRASILEIRA.2020,35(10):doi:10.1590/s0102-865020200100000002.
APA：
Xue Nan-Nan,He Miao,Li Yue,Wu Jun-Zhu,Du Wen-Wen...&Xiao Huai.(2020).Periplaneta americana extract promotes intestinal mucosa repair of ulcerative colitis in rat..ACTA CIRURGICA BRASILEIRA,35,(10)
MLA：
Xue Nan-Nan,et al."Periplaneta americana extract promotes intestinal mucosa repair of ulcerative colitis in rat.".ACTA CIRURGICA BRASILEIRA 35..10(2020)
