机构：[1]The First People’s Hospital of Yunnan Province, The Afliated Hospital of Kunming University of Science and Technology, No.157 Jinbi Road, Kunming 650032, Yunnan, China云南省第一人民医院
BackgroundIn past few years, long non-coding RNAs (lncRNAs) have been reported to play regulatory roles during cancer progression. LncRNA SNHG10 has been explored in several sorts of cancers. However, its detailed role and mechanism are still not well understood in glioma.MethodsExpression levels of genes were evaluated by RT-qPCR. EdU, TUNEL, sphere formation, wound healing and transwell assays appraised the effect of SNHG10 on glioma cellular processes. The interaction between molecules was examined by ChIP, RIP, RNA pull down and luciferase reporter assays.ResultsHigh level of SNHG10 was detected in glioma cells. Functional assay confirmed that SNHG10 promoted the proliferation, migration, invasion and stemness of glioma cells. Moreover, miR-532-3p was validated to bind with SNHG10 and expressed at a low level in glioma cells. Importantly, miR-532-3p exerted inhibitory functions in glioma. Furthermore, it was found that FBXL19 targeted by miR-532-3p facilitated cell growth and stemness in glioma, and that SNHG10 worked in glioma by increasing FBXL19 expression through sequestering miR-532-3p. More importantly, ETS1 promoted the transcription of SNHG10 and it mediated contribution to the malignant behaviors of glioma cells by SNHG10/miR-532-3p/FBXL19 signaling.ConclusionSNHG10 was transcriptionally activated by ETS1 and played an oncogenic role in glioma by sponging miR-532-3p and up-regulating FBXL19.
基金：
The help of all laboratory members in this study is appreciated.
第一作者机构：[1]The First People’s Hospital of Yunnan Province, The Afliated Hospital of Kunming University of Science and Technology, No.157 Jinbi Road, Kunming 650032, Yunnan, China
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推荐引用方式(GB/T 7714)：
Jin Lide,Huang Shengquan,Guan Congjin,et al.ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis[J].CANCER CELL INTERNATIONAL.2020,20(1):doi:10.1186/s12935-020-01649-2.
APA：
Jin, Lide,Huang, Shengquan,Guan, Congjin&Chang, Shun.(2020).ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis.CANCER CELL INTERNATIONAL,20,(1)
MLA：
Jin, Lide,et al."ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis".CANCER CELL INTERNATIONAL 20..1(2020)
