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Upregulation of long non-coding RNA RAB1A-2 induces FGF1 expression worsening lung cancer prognosis.

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机构: [1]The State Key Lab of Respiratory Disease, The First Affiliated Hospital, The institute for Chemical Carcinogenesis, Collaborative Innovation Center for Environmental Toxicity,Guangzhou Medical University, Guangzhou, 511436, China [2]Guangzhou Centre for Disease Control and Prevention, Guangzhou, 510440, China [3]Department of Central Laboratory, The 5th Affiliated Hospital of Guanzhou Medical University, Guangzhou, 510700, China. [4]School of Public health, Guangdong Medical University, Dongguan, 523808, China. [5]Center of Laboratory Animal, Guangzhou Medical University, Guangzhou, 511436, China [6]Yunnan Provence Tumor Hospital, the 3rd affiliated Hospital of the Kunming Medical University, Kunming, 650118, China [7]Department of Genetics, Medical College of Soochow University, Suzhou, 215006, China
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关键词: lung cancer lncRNA lnc-RAB1A-2 FGF1 survival

摘要:
The chromosomal locations of lncRNAs (long non-coding RNAs, lncRNAs) infer their biological functions in cancer. Lnc-RAB1A-2, a Ras-related protein Rab-1A (RAB1A) upstream lncRNA, was chosen for assessment of its impact on lung cancer prognosis in a case-based analysis and investigation of its biological function though a series of functional assays. Lnc-RAB1A-2 was significantly upregulated in 276 lung cancer tissues compared with corresponding non-tumor tissues, and its expression level was significantly correlated with clinical stage and metastasis status in lung cancer patients. Patients with high expression levels of this lncRNA had a shorter median survival time (16.0 months vs. 23.0 months, P = 0.011 in southern samples; 8.0 months vs. 19.0 months, P = 0.020 in eastern samples; 13.0 months vs. 19.0 months, P = 0.002 in merged samples) and a higher risk of death than those with lower levels (HR = 1.52; 95% CI = 1.01-2.26, in merged samples). Additionally, overexpression of lnc-RAB1A-2 significantly promoted lung cancer cell proliferation in vitro and in vivo. Further analyses using digital gene expression tag profiling revealed that lnc-RAB1A-2 could affect the expression of fibroblast growth factor 1 (FGF1), a gene involved in the PI3K/AKT/mTOR pathway that is largely activated by RAB1A. FGF1 was confirmed to be a down-stream gene of lnc-RAB1A-2. Collectively, our study demonstrated that lnc-RAB1A-2 is associated with poor lung cancer prognosis by promoting lung cancer development. Copyright © 2018 Elsevier B.V. All rights reserved.

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出版当年[2018]版:
大类 | 1 区 医学
小类 | 2 区 肿瘤学
最新[2023]版:
大类 | 1 区 医学
小类 | 2 区 肿瘤学
第一作者:
第一作者机构: [1]The State Key Lab of Respiratory Disease, The First Affiliated Hospital, The institute for Chemical Carcinogenesis, Collaborative Innovation Center for Environmental Toxicity,Guangzhou Medical University, Guangzhou, 511436, China [2]Guangzhou Centre for Disease Control and Prevention, Guangzhou, 510440, China
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通讯机构: [1]The State Key Lab of Respiratory Disease, The First Affiliated Hospital, The institute for Chemical Carcinogenesis, Collaborative Innovation Center for Environmental Toxicity,Guangzhou Medical University, Guangzhou, 511436, China [*1]The State Key Lab of Respiratory Disease,The First Affiliated Hospital, The Institute for Chemical Carcinogenesis, Collaborative Innovation Center for Environmental Toxicity, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, China
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