Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling.
机构：[1]The First Affiliated Hospital of Medical College, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China [2]Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China [3]Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China [4] The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China [5]Kunming Medical University, Kunming, Yunnan Province, China [6] Shanxi Medical University, Taiyuan, Shanxi Province, China
Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the di?erences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between sensory and motor nerves in Wallerian degeneration and the characteristics of peripheral nerve regeneration. The study was approved by the Ethics Committee of the Chinese PLA General Hospital, China (approval No. 2016-x9-07) in September 2016.
基金：
This study was supported by National Key Research & Development Program of China, No. 2016YFC11011601, 2017YFA0104701, the
Youth Cultivation Project of Military Medical Science, China, No. 15QNP091 (to YW), and People’s Liberation Army Youth Training Project for
Medical Science of China, No. 16QNP144 (to YW).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区医学
小类|3 区细胞生物学3 区神经科学
最新[2025]版：
大类|2 区医学
小类|2 区细胞生物学2 区神经科学
第一作者：
第一作者机构：[1]The First Affiliated Hospital of Medical College, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China [2]Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China [3]Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
共同第一作者：
通讯作者：
通讯机构：[2]Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China [3]Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
推荐引用方式(GB/T 7714)：
Wei Shuai,Liang Xue-Zhen,Hu Qian,et al.Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling.[J].Neural Regeneration Research.2020,15(2):315-323.doi:10.4103/1673-5374.265556.
APA：
Wei Shuai,Liang Xue-Zhen,Hu Qian,Wang Wei-Shan,Xu Wen-Jing...&Wang Yu.(2020).Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling..Neural Regeneration Research,15,(2)
MLA：
Wei Shuai,et al."Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling.".Neural Regeneration Research 15..2(2020):315-323
