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The effect of cyclic tensile force on the actin cytoskeleton organization and morphology of human periodontal ligament cells.

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机构: [1]Department of Orthodontics, School of Stomatology, Shandong University, Jinan, 250012, Shandong, China. [2]Department of the Oral and Maxillofacial Surgery, The Children's Hospital of Kunming, Kunming, 650500, Yunnan, China. [3]Department of Stomatology, The People's Hospital of Chuxiong Yi Autonomous Prefecture, Chuxiong, 675000, Yunnan, China. [4]Department of Orthodontics, Hospital of Stomatology, The First Hospital of Qujing, Qujing, 655000, Yunan, China. [5]Department of Orthodontics, School of Stomatology, Kunming Medical University, Kunming, 650500, Yunnan, China.
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关键词: Cyclic tensile force Periodontal ligament cells Orthodontic treatment Human girdin protein Transmission electron microscopic

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To explore Girdin/Akt pathway protein expression and morphology change by cyclic tension in the periodontal ligament cells. Human periodontal ligament cells were exposed to cyclic tension force at 4000 μstrain and 0.5 Hz for 6 h though a four-point bending system. Cyclic tension force upregulated F-actin, Girdin and Akt expression in hPDL. In transmission electron microscope assay showed that there are more and bigger mitochondria, more and longer cynapses, more cellular organisms after tension force stimulation than control. The actin filament was changed to be regular lines and pointed to poles of cells. However, we found that the Girdin-depleted cells are small and there are more micro-organisms including more lysosomes and matrix vesicles than control. These finding suggest that the STAT3/Girdin/Akt pathway in PDL to response to mechanical stimulation as well, and Girdin may play a significant role in triggering cell proliferation and migration during orthodontic treatment. It provided an insight into the molecular basis for development of a vitro cell model in studying orthodontic treatment. Copyright © 2018. Published by Elsevier Inc.

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出版当年[2018]版:
大类 | 3 区 生物
小类 | 3 区 生物物理 4 区 生化与分子生物学
最新[2023]版:
大类 | 3 区 生物学
小类 | 3 区 生物物理 4 区 生化与分子生物学
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第一作者机构: [1]Department of Orthodontics, School of Stomatology, Shandong University, Jinan, 250012, Shandong, China.
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