机构：[1]Department of Dermatology, The People’s Hospital of Baoshan, Baoshan, Yunnan, China[2]Department of Dermatology, The First Affiliated Hospital of Soochow University, Suzhou, China
Acne vulgaris (AV) is a chronic inflammatory disease of the pilosebaceous unit, and Propionibacterium acnes (P. acnes) has been implicated in acne inflammation. Numerous studies have shown that non-coding RNAs play important roles in regulating the pathophysiological processes of acne. In addition, the first imprinted long non-coding RNA (lncRNA) identified, H19, plays a critical role in inflammatory disease. However, the expression and role of H19 in AV remain unclear. In this study, we investigated the effects of H19 in keratinocytes and explored the regulatory mechanisms underlying these effects. H19 was upregulated in keratinocytes treated with P. acnes in a concentration-dependent manner. The phosphorylated forms of the nuclear factor (NF)-κB-related proteins IκBα (p-IκBα) and p65 (p-P65) were significantly upregulated after P. acnes treatment. Additionally, secretion of the proinflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 was upregulated in a concentration-dependent manner. Knockdown of H19 inhibited the expression of p-IκBα and p-P65 as well as the secretion of TNF-α, IL-6, and IL-8 in keratinocytes treated with P. acnes. Moreover, H19 was found to exert its proinflammatory effects by activating NF-κB. H19, which was localized mainly in the cytoplasm of keratinocytes, facilitated Toll-like receptor 2 (TLR2) expression by acting as a miR-196a sponge. H19 thus promoted the activation of NF-κB and the secretion of inflammatory cytokines through the miR-196a/TLR2 axis. These findings provide novel insight into the pathogenesis of AV.
基金：
This work was supported by the National Science
Foundation of China (Nos. 81960563 and 81760559).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区医学
小类|4 区细胞生物学4 区免疫学
最新[2023]版：
大类|2 区医学
小类|3 区细胞生物学3 区免疫学
第一作者：
第一作者机构：[1]Department of Dermatology, The People’s Hospital of Baoshan, Baoshan, Yunnan, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Dermatology, The First Affiliated Hospital of Soochow University, Suzhou, China[*1]Department of Dermatology, The First Affiliated Hospital of Soochow University, Suzhou, China
推荐引用方式(GB/T 7714)：
Yang Shuyun,Fang Fumin,Yu Xiuqin,et al.Knockdown of H19 Inhibits the Pathogenesis of Acne Vulgaris by Targeting the miR-196a/TLR2/NF-κB Axis.[J].Inflammation.2020,doi:10.1007/s10753-020-01268-z.
APA：
Yang Shuyun,Fang Fumin,Yu Xiuqin,Yang Changzhi,Zhang Xiaoping...&Zhu Tingting.(2020).Knockdown of H19 Inhibits the Pathogenesis of Acne Vulgaris by Targeting the miR-196a/TLR2/NF-κB Axis..Inflammation,,
MLA：
Yang Shuyun,et al."Knockdown of H19 Inhibits the Pathogenesis of Acne Vulgaris by Targeting the miR-196a/TLR2/NF-κB Axis.".Inflammation .(2020)
