机构：[1]Yunnan Provincial Key Laboratory of Vector‑borne Diseases Control and Research, Yunnan Provincial Center of Malaria Research, Yunnan Institute of Parasitic Diseases, Pu’er 665000, Yunnan, China.[2]School of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong, China.[3]Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Educa‑tion, The First Hospital of Jilin University, Changchun 130021, China.
Malaria is a major public health problem in the China-Myanmar border region. The genetic structure of malaria parasite may affect its transmission model and control strategies. The present study was to analyse genetic diversity of Plasmodium falciparum by merozoite surface proteins 1 and 2 (MSP1 and MSP2) and to determine the multiplicity of infection in clinical isolates in the China-Myanmar border region.
Venous blood samples (172) and filter paper blood spots (70) of P. falciparum isolates were collected from the patients of the China-Myanmar border region from 2006 to 2011. The genomic DNA was extracted, and the msp1 and msp2 genes were genotyped by nested PCR using allele-specific primers for P. falciparum.
A total of 215 P. falciparum clinical isolates were genotyped at the msp1 (201) and msp2 (204), respectively. For the msp1 gene, MAD20 family was dominant (53.49%), followed by the K1 family (44.65%), and the RO33 family (12.56%). For the msp2 gene, the most frequent allele was the FC27 family (80.93%), followed by the 3D7 family (75.81%). The total multiplicity of infection (MOI) of msp1 and msp2 was 1.76 and 2.21, with a prevalence of 64.19% and 72.09%, respectively. A significant positive correlation between the MOI and parasite density was found in the msp1 gene of P. falciparum. Sequence analysis revealed 38 different alleles of msp1 (14 K1, 23 MAD20, and 1 RO33) and 52 different alleles of msp2 (37 3D7 and 15 FC27).
The present study showed the genetic polymorphisms with diverse allele types of msp1 and msp2 as well as the high MOI of P. falciparum clinical isolates in the China-Myanmar border region.
基金：
National Research and Development Plan of
China (No. 2016YFC1200500).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|3 区医学
小类|2 区寄生虫学2 区热带医学3 区传染病学
最新[2023]版：
大类|3 区医学
小类|2 区热带医学3 区传染病学3 区寄生虫学
第一作者：
第一作者机构：[1]Yunnan Provincial Key Laboratory of Vector‑borne Diseases Control and Research, Yunnan Provincial Center of Malaria Research, Yunnan Institute of Parasitic Diseases, Pu’er 665000, Yunnan, China.
共同第一作者：
通讯作者：
通讯机构：[2]School of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong, China.[3]Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Educa‑tion, The First Hospital of Jilin University, Changchun 130021, China.
推荐引用方式(GB/T 7714)：
Zhang Cang-Lin,Zhou Hong-Ning,Liu Quan,et al.Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China-Myanmar border region.[J].Malaria journal.2019,18(1):367.doi:10.1186/s12936-019-3003-8.
APA：
Zhang Cang-Lin,Zhou Hong-Ning,Liu Quan&Yang Ya-Ming.(2019).Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China-Myanmar border region..Malaria journal,18,(1)
MLA：
Zhang Cang-Lin,et al."Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China-Myanmar border region.".Malaria journal 18..1(2019):367
