机构：[1]Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China,[2]Department of Orthopedics, The First Hospital of Kunming, Kunming, Yunnan, China,[3]Department of Orthopedics, The Affiliated Hospital of Hunan University of Medicine and The Third People’s Hospital of Huaihua, Huaihua, Hunan, China,[4]Department of Orthopedics, The People’s Hospital of Qingyuan, Qingyuan, Guangdong, China
Osteosarcoma is the most common primary bone malignancy in children and young adults. Increasing results suggest that discovery of microRNAs (miRNAs) might provide a novel therapeutical target for osteosarcoma.
MiR-182 expression level in osteosarcoma cell lines and tissues were assayed by qRT-PCR. MiRNA mimics or inhibitor were transfected for up-regulation or down-regulation of miR-182 expression. Cell function was assayed by CCK8, migration assay and invasion assay. The target genes of miR-182 were predicated by bioinformatics algorithm (TargetScan Human).
MiR-182 was down-regulated in osteosarcoma tissues and cell lines. Overexpression of miR-182 inhibited tumor growth, migration and invasion. Subsequent investigation revealed that TIAM1 was a direct and functional target of miR-182 in osteosarcoma cells. Overexpression of miR-182 impaired TIAM1-induced inhibition of proliferation and invasion in osteosarcoma cells.
Down-expression of miR-182 in osteosarcoma promoted tumor growth, migration and invasion by targeting TIAM1. MiR-182 might act as a tumor suppressor gene whose down-regulation contributes to the progression and metastasis of osteosarcoma, providing a potential therapy target for osteosarcoma patients.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|3 区生物
小类|3 区综合性期刊
最新[2025]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
第一作者：
第一作者机构：[1]Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China,[2]Department of Orthopedics, The First Hospital of Kunming, Kunming, Yunnan, China,
通讯作者：
推荐引用方式(GB/T 7714)：
Jun Hu,Guohua Lv,Shuguang Zhou,et al.The Downregulation of MiR-182 Is Associated with the Growth and Invasion of Osteosarcoma Cells through the Regulation of TIAM1 Expression.[J].PloS one.2015,10(5):e0121175.doi:10.1371/journal.pone.0121175.
APA：
Jun Hu,Guohua Lv,Shuguang Zhou,Yucheng Zhou,Bangxu Nie...&Xiaofeng Yuan.(2015).The Downregulation of MiR-182 Is Associated with the Growth and Invasion of Osteosarcoma Cells through the Regulation of TIAM1 Expression..PloS one,10,(5)
MLA：
Jun Hu,et al."The Downregulation of MiR-182 Is Associated with the Growth and Invasion of Osteosarcoma Cells through the Regulation of TIAM1 Expression.".PloS one 10..5(2015):e0121175
