机构：[1]Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.[2]AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.[3]Infectious Diseases Department, Beijing Youan Hospital, Capital Medical University, Beijing, China.[4]Sexually transmitted Disease and AIDS Department, Anhui Provincial Center for Disease Control and Prevention, Hefei, China.[5]Yunnan Provincial Center for Disease Control and Prevention, Kunming, China.[6]AIDS/STIs Prevention and Control Department, Hunan Provincial Center for Disease Control and Prevention, Changsha, China.[7]Sichuan provincial center for disease control and prevention, Chengdu, China.[8]Henan Provincial Center for Disease Control and Prevention, Zhengzhou, China.[9]Sexually Transmitted Disease and AIDS Prevention and Control Department, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.[10]Sexually transmitted Disease and AIDS Department, Shandong Provincial Center for Disease Control and Prevention, Jinan, China.[11]Infectious Disease Department, Guangzhou No. 8 Renmin Hospital, Guangzhou, China.[12]Sexually transmitted Disease and AIDS Department, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.[13]Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.
The HIV-1 epidemic among men-who-have-sex-with-men (MSM) continues to expand in China, involving the co-circulation of several different lineages of HIV-1 strains, including subtype B and CRF01_AE. This expansion has created conditions that facilitate the generation of new recombinant strains. A molecular epidemiologic survey among MSM in 11 provinces/cities around China was conducted from 2008 to 2013. Based on pol nucleotide sequences, a total of 19 strains (1.95%) belonged to the CRF55_01B were identified from 975 MSM in 7 provinces, with the prevalence range from 1.5% to 12.5%. Near full length genome (NFLG) sequences from six epidemiologically-unlinked MSM were amplified for analyzing evolutionary history, an identical genome structure composed of CRF01_AE and subtype B with four unique recombination breakpoints in the pol region were identified. Bayesian molecular clock analyses for both CRF01_AE and B segments indicated that the estimated time of the most recent common ancestors of CRF55_01B was around the year 2000. Our study found CRF55_01B has spread throughout the most provinces with high HIV-1 prevalence and highlights the importance of continual surveillance of dynamic changes in HIV-1 strains, the emergence of new recombinants, and the need for implementing effective prevention measures specifically targeting the MSM population in China.
基金：
Mega-projects of National Science Research for the 12th Five-Year Plan (2012ZX10001-
006),“Development plan of innovative group” of Ministry of Education 2012 and National natural science
foundation (81371787)
第一作者机构：[1]Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.
通讯作者：
通讯机构：[1]Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.[2]AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
推荐引用方式(GB/T 7714)：
Xiaoxu Han,Yutaka Takebe,Weiqing Zhang,et al.A Large-scale Survey of CRF55_01B from Men-Who-Have-Sex-with-Men in China: implying the Evolutionary History and Public Health Impact[J].SCIENTIFIC REPORTS.2015,5:doi:10.1038/srep18147.
APA：
Xiaoxu Han,Yutaka Takebe,Weiqing Zhang,Minghui An,Bin Zhao...&Hong Shang.(2015).A Large-scale Survey of CRF55_01B from Men-Who-Have-Sex-with-Men in China: implying the Evolutionary History and Public Health Impact.SCIENTIFIC REPORTS,5,
MLA：
Xiaoxu Han,et al."A Large-scale Survey of CRF55_01B from Men-Who-Have-Sex-with-Men in China: implying the Evolutionary History and Public Health Impact".SCIENTIFIC REPORTS 5.(2015)
