机构：[1]Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, No. 100, Xianlie Zhong Road, Guangzhou, Guangdong 510070, People’s Republic of China.[2]Faculty Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, Yunnan, China.[3]Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, People’s Republic of China.
Human noroviruses are one of the main causes of foodborne illnesses and represent a serious public health concern. Rapid and sensitive assays for human norovirus detection are undoubtedly necessary for clinical diagnosis, especially in regions without more sophisticated equipment.
The rapid reverse transcription recombinase-aided amplification (RT-RAA) is a fast, robust and isothermal nucleic acid detection method based on enzyme reaction. This method can complete the sample detection at 39 °C in 30 min. In this study, we successfully established a rapid reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of human norovirus GII.4 and applied this assay to clinical samples, as well as comparison with commercial reverse transcription real-time fluorescence quantitative PCR (RT-qPCR).
At 95% probability, the detection sensitivity of RT-RAA was 3.425 log10 genomic copies (LGC)/reaction. Moreover, no cross-reaction was observed with other norovirus genogroups and other common foodborne viruses. Stool samples were examined by RT-RAA and reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Compared of RT-qPCR, kappa values for human norovirus detection with RT-RAA were 0.894 (p < 0.001), indicating that both assays were in agreement.
This RT-RAA assay provides a rapid, specific, and sensitive assay for human norovirus detection and is suitable for clinical testing.
基金：
This work was supported by the National Key Research and Development
Program of China (2018YFC1602500), the National Natural Science
Foundation of China (31872912), the Natural Science Foundations of
Guangdong Province for Distinguished Young Scholars (2019B151502065),
the Key Research and Development Program of Guangdong Province
(2019B020209001), and GDAS’Project of Science and Technology
Development (2020GDASYL-20200104008).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|3 区医学
小类|4 区传染病学
最新[2023]版：
大类|3 区医学
小类|3 区传染病学
第一作者：
第一作者机构：[1]Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, No. 100, Xianlie Zhong Road, Guangzhou, Guangdong 510070, People’s Republic of China.[2]Faculty Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, Yunnan, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Qin Zhiwei,Xue Liang,Cai Weicheng,et al.Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4.[J].BMC infectious diseases.2021,21(1):248.doi:10.1186/s12879-021-05942-x.
APA：
Qin Zhiwei,Xue Liang,Cai Weicheng,Gao Junshan,Jiang Yueting...&Wu Qingping.(2021).Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4..BMC infectious diseases,21,(1)
MLA：
Qin Zhiwei,et al."Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4.".BMC infectious diseases 21..1(2021):248
