机构：[1]Department of Orthopedic Surgery, The First People's Hospital of Yunnan province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, China.外科片骨科云南省第一人民医院[2]Faculty of Medical Science, Kunming University of Science and Technology, Kunming 650500, China.[3]Yunnan Key Laboratory of Digital Orthopaedics, The First People's Hospital of Yunnan province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, China.外科片骨科云南省第一人民医院[4]Department of orthopedics, Yan'an Hospital, Kunming Medical University, Kunming 650035, China.
This study explored the molecular mechanism behind the protective effects from low-dose lipopolysaccharide (LPS) on an in-vitro model of spinal cord injury (SCI). For this, PC12 cells were treated with different concentrations of LPS and the cell counting kit-8 assay was used to measure the toxicity of LPS to the cells. Next, we used immunofluorescence to measure nuclear translocation of Nrf2 in PC12 cells. PC12 cells were then treated with IGF-1 (PI3K agonist) and LY294002 (PI3K inhibitor). An in-vitro model of SCI was then established via oxygen-glucose deprivation/reoxygenation. Rates of apoptosis were measured using flow cytometry and the TUNEL assay. Low-dose LPS increased the expression levels of Nrf2, p-PI3K/PI3K, and p-AKT/AKT, and facilitated nuclear translocation of Nrf2. The activation of PI3K-AKT signaling by IGF-1 significantly increased the expression of Nrf2, whereas inhibition of PI3K-AKT signaling significantly decreased the expression of Nrf2. Low-dose LPS reduced the apoptotic ratio of PC12 cells, decreased the expression levels of caspase 3 and caspase 9, and increased the expression levels of HO-1, NQO1, and γ-GCS. Low-dose LPS also reduced the rate of apoptosis and oxidative stress by activating the PI3K-AKT-Nrf2 signaling pathway. Collectively, the results indicate that PI3K-AKT-Nrf2 signaling participates in the protective effects from low-dose LPS in an in-vitro PC12 cell model of SCI.
基金：
National Natural Science
Foundation of China (grant No. 81860240), Basic Research Project of
Yunnan Provincial Science and Technology (grant No. 2018FB119),
Yunnan Provincial Science and Technology Department-Kunming
Medical University Joint Special Project (grant No. 2019FE001–123),
and Yunnan Health Training Project of High Level Talents
(grant No. H-2018100).
第一作者机构：[1]Department of Orthopedic Surgery, The First People's Hospital of Yunnan province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, China.[2]Faculty of Medical Science, Kunming University of Science and Technology, Kunming 650500, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Li Wei-Chao,Yao Shao-Ping,Zhang Jun,et al.Low-dose lipopolysaccharide protects nerve cells against spinal cord injury via regulating the PI3K-AKT-Nrf2 signaling pathway.[J].BIOCHEMISTRY AND CELL BIOLOGY.2021,99(5):527-535.doi:10.1139/bcb-2020-0641.
APA：
Li Wei-Chao,Yao Shao-Ping,Zhang Jun,Liu Wei-Bing,Liu Jie&Geng Cheng-Kui.(2021).Low-dose lipopolysaccharide protects nerve cells against spinal cord injury via regulating the PI3K-AKT-Nrf2 signaling pathway..BIOCHEMISTRY AND CELL BIOLOGY,99,(5)
MLA：
Li Wei-Chao,et al."Low-dose lipopolysaccharide protects nerve cells against spinal cord injury via regulating the PI3K-AKT-Nrf2 signaling pathway.".BIOCHEMISTRY AND CELL BIOLOGY 99..5(2021):527-535
