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MiR-9-5p promotes M1 cell polarization in osteoarthritis progression by regulating NF-κB and AMPK signaling pathways by targeting SIRT1.

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收录情况： ◇ SCIE

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机构： [1]Department of Rheumatology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China [2]The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China

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To investigate the roles and regulatory mechanisms of miR-9-5p in the development of osteoarthritis (OA).Synovial tissues from mouse OA model and control groups were collected and miR-9-5p expression levels and macrophage markers were measured with qPCR. The function of miR-9-5p in macrophage polarization was analyzed by flow cytometry and qPCR. Various databases were employed to screen the target genes one of which was validated with dual-luciferase analysis. Following the validation, rescue research was applied, and the signaling pathways were analyzed with Western blotting. Finally, the role of miR-9-5p in the progression of OA was validated in the mouse model.MiR-9-5p was highly expressed in the synovial tissues of the OA model and was positively associated with M1 markers. Function analysis demonstrated that miR-9-5p could promote the progression of OA by promoting M1 polarization and inhibiting M2 polarization in vivo and in vitro. The mechanism analysis demonstrated that miR-9-5p could regulate macrophage polarization via NF-κB and AMPK signaling pathways by inhibiting SIRT1 expression.MiR-9-5p could promote M1 polarization and OA progression by regulating NF-κB and AMPK signaling pathways by inhibiting SIRT1 expression.Copyright © 2021 Elsevier B.V. All rights reserved.

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出版当年[2021]版：

大类 | 2 区医学

小类 | 2 区药学 3 区免疫学

最新[2023]版：

大类 | 2 区

医学

小类 | 2 区免疫学 2 区药学

JCR分区：

出版当年[2020]版：

Q2 IMMUNOLOGY Q2 PHARMACOLOGY & PHARMACY

最新[2023]版：

Q1 PHARMACOLOGY & PHARMACY Q2 IMMUNOLOGY

影响因子： 4.8 最新[2023版] 5 最新五年平均 4.932 出版当年[2020版] 4.624 出版当年五年平均 3.943 出版前一年[2019版] 5.714 出版后一年[2021版]

第一作者：

第一作者机构： [1]Department of Rheumatology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China [2]The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China

通讯作者：

通讯机构： [1]Department of Rheumatology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China [2]The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China [*1]Department of Rheumatology, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, No. 157 Jinbi Road, Kunming 650032, Yunnan, China.

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