The cardiovascular is a system that contains extremely complex mechanical factors, in which the circulatory flow of blood has rich mechanical laws. Many studies have revealed that mechanical factors play a very important role in the process of revascularization. Hence, it is essential to investigate the mechanical factors in the process of revascularization in depth. A cyclic tensile strain (CTS) was applied to human aortic smooth muscle cells (HASMCs) at a frequency of 1 Hz and amplitudes of 5%, 10% and 15%, respectively. SmallRNA-seq was used to identify differentially expressed miRNAs (DE-miRNAs) responding to CTS in HASMCs. Starbase database predicted the target genes of DE-miRNAs. Metascape was applied for GO and KEGG pathway enrichment analysis and protein-protein interaction network construction. The proliferation and migration of CTS-treated HASMCs were significantly enhanced, and apoptosis were significantly reduced compared to the control group. SmallRNA-seq results demonstrated that 55, 16 and 16 DE-miRNAs were present in 5%, 10% and 15% CTS-treated HASMCs, respectively. Compared to controls, with miR-26a-2-3p and miR-187-3p being the intersection of these DE-miRNAs. Starbase database identified 189 common target genes for miR-26a-2-3p and miR-187-3p. Common target genes are mainly enriched in the basolateral plasma membrane and endocytosis. Further, in vitro experiments exhibited that CTS upregulated miR-187-3p expression, and miR-187-3p enhanced the proliferation and migration of HASMCs and reduced their apoptosis. It is suggested that miR-187-3p may be an important target for CTS participate in the process of cardiovascular disease. [GRAPHICS]
基金：
[National Natural Science Foundation of China 81760092]; [81860174]; [Yunnan Provincial Clinical Medical Center of Cardio-cerebral Vascular Diseases ZX2019-03-01].
基金编号：National Natural Science Foundation of China 8176009281860174Yunnan Provincial Clinical Medical Center of Cardio-cerebral Vascular Diseases ZX2019-03-01
第一作者机构：[1]Kunming Med Univ, Dept Ophthalmol, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[2]Kunming Med Univ, Dept Vasc Surg, Affiliated Hosp 1, 295 XiChang Rd, Kunming, Yunnan, Peoples R China[*1]Department of Vascular Surgery, The First Affiliated Hospital of Kunming Medical University, No. 295 Xi’Chang Road, Kunming, Yunnan, China
推荐引用方式(GB/T 7714)：
Yang Di,Wei Guang-Yuan,Li Min,et al.Cyclic tensile strain facilitates proliferation and migration of human aortic smooth muscle cells and reduces their apoptosis via miRNA-187-3p[J].BIOENGINEERED.2021,12(2):11439-11450.doi:10.1080/21655979.2021.2009321.
APA：
Yang, Di,Wei, Guang-Yuan,Li, Min,Peng, Ming-Sheng,Sun, Yuan...&Cai, Hong-Bo.(2021).Cyclic tensile strain facilitates proliferation and migration of human aortic smooth muscle cells and reduces their apoptosis via miRNA-187-3p.BIOENGINEERED,12,(2)
MLA：
Yang, Di,et al."Cyclic tensile strain facilitates proliferation and migration of human aortic smooth muscle cells and reduces their apoptosis via miRNA-187-3p".BIOENGINEERED 12..2(2021):11439-11450
