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LncRNA MALAT1 aggravates the retinal angiogenesis via miR-320a/HIF-1α axis in diabetic retinopathy.

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机构： [1]Department of Ophthalmology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, China [2]Medical College of Kunming University of Science and Technology, Kunming, Yunnan, 650500, China [3]Medical College of Dali University, Dali, Yunnan, 671003, China

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Diabetic retinopathy (DR) is one of the most serious microvascular complications of diabetes and an important cause of blindness in adults. In previous study, we found that miR-320a alleviated the damage of muller cells in DR. In this study, we mainly explored the mechanism of lncRNA MALAT1 on retinal angiogenesis in DR by regulating miR-320a/HIF-1α. The expression of MALAT1 and miR-320a was detected by RT-qPCR, and the expression of HIF-1α was detected by Western blot. The superoxide anion level, invasion, angiogenesis, and vascular permeability of mouse retinal microvascular endothelial cells (MRMECs) co-cultured with muller cells were evaluated by dihydroethidium, transwell, angiogenesis and immunofluorescence assay. In order to analyze the targeting relationship between miR-320a and MALAT1 or HIF-1α, we performed dual luciferase reporter gene, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP) and RNA pulldown experiments. The results should that MALAT1 and HIF-1α were highly expressed and miR-320a was low expressed in high glucose (HG)-induced muller cells, and MALAT1 could competitively bind with HIF-1α. Knocking down miR-320a inhibited MRMECs invasion angiogenesis, and vascular permeability by targeting miR-320a. Overexpression of miR-320a down regulated HIF-1α and inhibited the invasion, angiogenesis, and vascular permeability of MRMECs. In conclusion, MALAT1 inhibits HIF-1α expression and MRMECs angiogenesis in DR through spongy miR-320a.Copyright © 2022. Published by Elsevier Ltd.

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出版当年[2022]版：

大类 | 3 区医学

小类 | 2 区眼科学

最新[2023]版：

大类 | 2 区医学

小类 | 2 区眼科学

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出版当年[2021]版：

Q2 OPHTHALMOLOGY

最新[2023]版：

Q1 OPHTHALMOLOGY

影响因子： 3 最新[2023版] 3.3 最新五年平均 3.77 出版当年[2021版] 3.952 出版当年五年平均 3.467 出版前一年[2020版] 3.4 出版后一年[2022版]

第一作者：

第一作者机构： [1]Department of Ophthalmology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, China [2]Medical College of Kunming University of Science and Technology, Kunming, Yunnan, 650500, China [3]Medical College of Dali University, Dali, Yunnan, 671003, China

通讯作者：

通讯机构： [1]Department of Ophthalmology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, 650032, China [*1]Department of ophthalmology, The First People’s Hospital of Yunnan Province, 157 Jinbi Road, Xishan District, Kunming, Yunnan, 650032, China

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