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LncRNA SENCR overexpression attenuated the proliferation, migration and phenotypic switching of vascular smooth muscle cells in aortic dissection via the miR-206/myocardin axis.

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收录情况： ◇ SCIE

作者：
Song Yi[1] * ; Wang Tao[2] * ; Mu Chunjie[3] ; Gui Wenting[3] ; Deng Yao[3] ; Ma Runwei[3,*1] ;
机构： [1]Department of Extracorporeal Circulation, Fuwai Yunnan Cardiovascular Hospital, Kunming, 650102, China [2]Department of Cardiothoracic Surgery, The First People’s Hospital of Kunming, Kunming, 650034, China [3]Department of Cardiovascular Surgery, Fuwai Yunnan Cardiovascular Hospital, Kunming, 650102, China
出处：
DOI： 10.1016/j.numecd.2022.03.004
ISSN：

摘要：
Smooth muscle and endothelial cell-enriched migration/differentiation-associated lncRNA (SENCR) has been reported to be associated with some cardiovascular diseases; however, its function and exact molecular mechanism in aortic dissection (AD) remain undefined. Thus, we investigated the effects of SENCR on AD and its potential mechanisms.SENCR expression in aortic media specimens from AD patients was detected by quantitative real-time PCR (qPCR). The roles of SENCR in vascular smooth muscle cell (VMSC) proliferation and migration as well as in the regulation of contractile phenotype genes were studied using CCK-8, wound healing, Transwell, qPCR and Western blot assays. Dual-luciferase reporter assays were performed to identify the regulatory correlation between SENCR, miR-206 and myocardin. Furthermore, mouse AD models were constructed with ApoE-/- mice, and the effect of upregulated SENCR on phenotypic switching in the AD model was detected using hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) assays. SENCR overexpression inhibited VSMC proliferation, migration and synthetic phenotype-related gene expression; decreased miR-206 expression; increased myocardin expression; and suppressed rupture of the aortic media in mice. SENCR knockdown had the opposite effects. Our results further suggested that miR-206 upregulation could reverse the inhibitory roles of SENCR upregulation and that myocardin upregulation could restore the function of SENCR upregulation in VSMCs. Dual-luciferase reporter assays confirmed that SENCR regulated miR-206, which directly targeted myocardin in VSMCs.SENCR overexpression suppressed VMSC proliferation and migration, maintained the contractile phenotype and suppressed aortic dilatation via the miR-206/myocardin axis.Copyright © 2022 The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved.

基金：
This work was supported by the Open Project of the State Key Laboratory of Cardiovascular Diseases [NO. 2019kfyf- 05] and the Association Foundation Program of Yunnan Provincial Science and Technology Department and Kunming Medical University [NO. 202001AY070001-106, 202001AY070001-009].
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外文
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中科院(CAS)分区：
出版当年[2022]版：
大类 | 3 区 医学
小类 | 3 区 营养学 3 区 内分泌学与代谢 3 区 心脏和心血管系统
最新[2023]版：
大类 | 3 区 医学
小类 | 3 区 心脏和心血管系统 3 区 内分泌学与代谢 3 区 营养学
JCR分区：
出版当年[2021]版：
Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Q2 ENDOCRINOLOGY & METABOLISM Q2 NUTRITION & DIETETICS
最新[2023]版：
Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Q2 ENDOCRINOLOGY & METABOLISM Q2 NUTRITION & DIETETICS

影响因子： 最新[2023版] 最新五年平均 出版当年[2021版] 出版当年五年平均 出版前一年[2020版] 出版后一年[2022版]

第一作者：
第一作者机构： [1]Department of Extracorporeal Circulation, Fuwai Yunnan Cardiovascular Hospital, Kunming, 650102, China
共同第一作者：
通讯作者：
通讯机构： [3]Department of Cardiovascular Surgery, Fuwai Yunnan Cardiovascular Hospital, Kunming, 650102, China [*1]Department of Cardiovascular Surgery, Fuwai Yunnan Cardiovascular Hospital, NO. 528, Shahe North Road, Kunming, 650102, China.
推荐引用方式(GB/T 7714)：
Song Yi,Wang Tao,Mu Chunjie,et al.LncRNA SENCR overexpression attenuated the proliferation, migration and phenotypic switching of vascular smooth muscle cells in aortic dissection via the miR-206/myocardin axis.[J].NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES.2022,32(6):1560-1570.doi:10.1016/j.numecd.2022.03.004.
APA：
Song Yi,Wang Tao,Mu Chunjie,Gui Wenting,Deng Yao&Ma Runwei.(2022).LncRNA SENCR overexpression attenuated the proliferation, migration and phenotypic switching of vascular smooth muscle cells in aortic dissection via the miR-206/myocardin axis..NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES,32,(6)
MLA：
Song Yi,et al."LncRNA SENCR overexpression attenuated the proliferation, migration and phenotypic switching of vascular smooth muscle cells in aortic dissection via the miR-206/myocardin axis.".NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES 32..6(2022):1560-1570

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