机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan Province, China[2]Medical Faculty of Kunming University of Science and Technology, Kunming, Yunnan Province, China[3]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province, China云南省第一人民医院[4]Department of Rheumatology, the First People’s Hospital of Yunnan Province, Kunming, China内科片风湿免疫科云南省第一人民医院
Increased proliferation and impaired death of fibroblast-like synovial cells play an important role in the development of rheumatoid arthritis (RA). Survivin plays an important role in the prodromal stage and prognosis of RA and has been introduced as a biomarker of joint injury in RA patients. The purpose of this study was to explore whether propionate alleviates RA through miR-140-5p/survivin pathway.The synovial tissues of RA patients were collected to detect the expression levels of miR-140-5p and survivin; normal human fibroblast-like synovial cells (HLSs) and RA fibroblast-like synovial cells (RA-FLSs) were cultured and treated with 10 mM of sodium propionate (SP), then the expressions of miR-140-5p and survivin, cell viability and apoptosis were detected; collagen induced arthritis (CIA) rat model was constructed and treated with SP, then the tissue inflammation level and the expression levels of miR-140-5p and Survivin were detected.The expression of miR-140-5p decreased in synovial tissues of RA patients and RA-FLSs cells, while the expression of survivin increased significantly in RA patients. SP promoted miR-140-5p expression and apoptosis in RA-FLSs cells and inhibited survivin expression and cell viability of RA-FLSs cells. In addition, miR-140-5p plays a protective role by targeting survivin. Importantly, in the CIA rat model, SP reduced joint inflammatory response, and the miR-140-5p inhibitor weakened the protective effect of SP.SP can alleviate RA by promoting the expression of miR-140-5p and inhibiting the excessive proliferation and death impairment of RA-FLSs cells induced by survivin.
基金：
Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation [2019FE001(-283)]
第一作者机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan Province, China[2]Medical Faculty of Kunming University of Science and Technology, Kunming, Yunnan Province, China[3]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province, China[4]Department of Rheumatology, the First People’s Hospital of Yunnan Province, Kunming, China
通讯作者：
通讯机构：[1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan Province, China[2]Medical Faculty of Kunming University of Science and Technology, Kunming, Yunnan Province, China[3]The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province, China[4]Department of Rheumatology, the First People’s Hospital of Yunnan Province, Kunming, China[*1]No. 157 Jinbi Road, Xishan District, Kunming, 650032, Yunnan, China
推荐引用方式(GB/T 7714)：
Ma Sha,Wang Jing,He Fang,et al.Sodium propionate improves rheumatoid arthritis by inhibiting survivin mediated proliferation of fibroblast like synoviocytes by promoting miR-140-5p.[J].AUTOIMMUNITY.2022,55(6):378-387.doi:10.1080/08916934.2022.2073589.
APA：
Ma Sha,Wang Jing,He Fang,Zuo Dachen,Li Fayou...&Li Qin.(2022).Sodium propionate improves rheumatoid arthritis by inhibiting survivin mediated proliferation of fibroblast like synoviocytes by promoting miR-140-5p..AUTOIMMUNITY,55,(6)
MLA：
Ma Sha,et al."Sodium propionate improves rheumatoid arthritis by inhibiting survivin mediated proliferation of fibroblast like synoviocytes by promoting miR-140-5p.".AUTOIMMUNITY 55..6(2022):378-387
