机构：[1]Department of Ophthalmology, The First People's Hospital of Yunnan, Kunming, Yunnan, China[2]Department of Ophthalmology, Research Center of Fundus Disease of Yunnan, Kunming, Yunnan, China[3]Department of Ophthalmology, Kunming University of Science and Technology Affiliated Hospital, Kunming, Yunnan, China[4]Medical college, Kunming University of Science and Technology, Kunming, Yunnan, China[5]5 Department of Ophthalmology, Dali University, Dali, Yunnan, China[6]Head and Neck Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Müller cells are closely related to diabetic retinopathy (DR). Aquaporin‐4(AQP4) can effectively promote the diffusion of water across cellular membranes. However, the dynamic balance of water plays key role in many diseases, such as cerebral edema. Meanwhile, the unusual expression and distribution of AQP4 in the retina are the significant causes of ocular hypertension and reperfusion injury. To explore the functional significance between microRNA‐320a (miR‐320a) and AQP4 in pathological hypoxia‐induced DR related retinal edema, we hypothesized that miR‐320a regulates AQP4 expression and internalization to relieve the edema of Müller cells under the pathological retinal hypoxia stress by targeting AQP4, thereby attenuate the damage of Müller cells. Results demonstrated that miR‐320a mimics inhibited the expressions of AQP4 in Müller cells. Furthermore, overexpression miR‐320a protected Müller cells by suppressing superoxide anion.In addition, overexpression miR‐320a markedly attenuated hypoxia‐induced injury, significantly increased the cell viability, and promoted the internalization of AQP4. Furthermore, miR‐320a can also regulate the stable anchoring of AQP4 on the cell membrane. Our study indicated that miR‐320a may be a potential modulator which can mediate AQP4 expression and attenuate the hypoxia damage of Müller cells. In conclusion, miR‐320a may be a potential target for DR therapy by targeting AQP4.
基金：
Joint project of Yunnan science and technology department‐ Kunming medical university Applied Fundamental Research; The Applied Basic Research Key Project of Yunnan Provincial Fundus Disease Research Center; Science and Technology Talents and Platform Project (Academician Workstation of Zhengqin Yin; National Natural Science Foundation of China
第一作者机构：[1]Department of Ophthalmology, The First People's Hospital of Yunnan, Kunming, Yunnan, China[2]Department of Ophthalmology, Research Center of Fundus Disease of Yunnan, Kunming, Yunnan, China[3]Department of Ophthalmology, Kunming University of Science and Technology Affiliated Hospital, Kunming, Yunnan, China[4]Medical college, Kunming University of Science and Technology, Kunming, Yunnan, China[5]5 Department of Ophthalmology, Dali University, Dali, Yunnan, China
通讯作者：
通讯机构：[1]Department of Ophthalmology, The First People's Hospital of Yunnan, Kunming, Yunnan, China[2]Department of Ophthalmology, Research Center of Fundus Disease of Yunnan, Kunming, Yunnan, China[3]Department of Ophthalmology, Kunming University of Science and Technology Affiliated Hospital, Kunming, Yunnan, China[4]Medical college, Kunming University of Science and Technology, Kunming, Yunnan, China[5]5 Department of Ophthalmology, Dali University, Dali, Yunnan, China
推荐引用方式(GB/T 7714)：
Chen Zhen,Zhengrong Yang,Xiaoliang Li,et al.microRNA‐320a prevent Müller cells from hypoxia injury by targeting aquaporin‐4[J].Journal of cellular Biochemistry.2020,121(12):1-13.doi:10.1002/jcb.29524.
APA：
Chen Zhen,Zhengrong Yang,Xiaoliang Li,He Wang,Yonggang Wang...&mei Y.(2020).microRNA‐320a prevent Müller cells from hypoxia injury by targeting aquaporin‐4.Journal of cellular Biochemistry,121,(12)
MLA：
Chen Zhen,et al."microRNA‐320a prevent Müller cells from hypoxia injury by targeting aquaporin‐4".Journal of cellular Biochemistry 121..12(2020):1-13
