机构：[1]Department of Medical Oncology, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China.[2]Department of Gastroenterology, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China
BACKGROUNDWe previously demonstrated that heat shock factor protein 4 (HSF4) facilitates colorectal cancer (CRC) progression. DNA methylation, a major modifier of gene expression and stability, is involved in CRC development and outcome.AIMTo investigate the correlation between HSF4 methylation and CRC risk, and to uncover the underlying molecular mechanisms.METHODSDifferences in beta values of HSF4 methylation loci in multiple malignancies and their correlation with HSF4 mRNA expression were analyzed based on Shiny Methylation Analysis Resource Tool. HSF4 methylation-related genes were identified by LinkedOmics in CRC, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed. Protein-protein interaction network of HSF4 methylation-related genes was constructed by String database and MCODE algorithm.RESULTSA total of 19 CpG methylation loci were identified in HSF4, and their beta values were significantly increased in CRC tissues and exhibited a positive correlation with HSF4 mRNA expression. Unfortunately, the prognostic and diagnostic performance of these CpG loci in CRC patients was mediocre. In CRC, there were 1694 HSF4 methylation-related genes; 1468 of which displayed positive and 226 negative associations, and they were involved in regulating phenotypes such as immune, inflammatory, and metabolic reprogramming. EGFR, RELA, STAT3, FCGR3A, POLR2K, and AXIN1 are hub genes among the HSF4 methylation-related genes.CONCLUSIONHSF4 is highly methylated in CRC, but there is no significant correlation between it and the prognosis and diagnosis of CRC. HSF4 methylation may serve as one of the ways in which HSF4 mediates the CRC process.
基金：
National Natural Science Foundation of China [82260601]; Joint Foundation of Kunming Medical University and Yunnan Provincial Science and Technology Department [202201AY070001-256]; Grant for Clinical Medical Center of Yunnan Provincial Health Commission [2021LCZXXF-XH03]; Young Academic Talents Cultivation Foundation of Yunnan Province [202205AC160070]
第一作者机构：[1]Department of Medical Oncology, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Wenjing,Yue Kelin,Wang jingzhai,et al.Association between heat shock factor protein 4 methylation and colorectal cancer risk and potential molecular mechanisms: A bioinformatics study[J].World J Gastrointest Oncol.2023,15(12):2150-2168.doi:10.4251/wjgo.v15.i12.2150.
APA：
Zhang Wenjing,Yue Kelin,Wang jingzhai&Zhang Yu.(2023).Association between heat shock factor protein 4 methylation and colorectal cancer risk and potential molecular mechanisms: A bioinformatics study.World J Gastrointest Oncol,15,(12)
MLA：
Zhang Wenjing,et al."Association between heat shock factor protein 4 methylation and colorectal cancer risk and potential molecular mechanisms: A bioinformatics study".World J Gastrointest Oncol 15..12(2023):2150-2168
