机构：[1]Department of Emergency, the First People’s Hospital of Yunnan Province,the Affiliated Hospital of Kunming University of Science and Technology, Xishan District, No.157 Jinbi Road Yunnan Province, Kunming City, China门急诊片急诊内科云南省第一人民医院[2]Department of Basic Research Institute, the First People’s Hospital of Yunnan Province, the Affiliated Hospital of Kunming University of Science and Technology,Xishan District, No.157 Jinbi Road Yunnan Province, Kunming City, China云南省第一人民医院[3]To whom correspondence should be addressed at Department of Emergency, the First People’s Hospital of Yunnan Province,the Affiliated Hospital of Kunming University of Science and Technology, Xishan District, No.157 Jinbi Road Yunnan Province, Kunming City, China. Email: he_ quan_2009@126.com
WIN 55212-2 is an endocannabinoids analogue that has been reported to have anti-inflammatory and anti-fibrosis effects on different models. In this study, we investigated the protective effects of WIN 55212-2 on paraquat (PQ)-induced poison on mice especially on lung injury. Mice were administrated with different dose of PQ and thereafter treated with 0.2 mg/kg or 1 mg/kg WIN 55212-2. The survival of mice was recorded during 4 weeks of observation. Twenty-eight days after PQ treatment, the cell population and inflammatory factors IL-6, IL-10, and TNF-alpha were measured in bronchoalveolar lavage fluid (BALF). Pulmonary fibrosis was evaluated by Masson staining. Our results showed that WIN 55212-2 treatment reduced PQ-induced mortality of mice in a dose dependent manner. It decreased the number of inflammation-associated cells, as well as the level of pro-inflammatory factors in BALF (P < 0.05). WIN 55212-2 increased M2 cells in BALF (P < 0.05), improved the lung histology, reduced fibrosis formation, and decreased TGF-beta, alpha-SMA and PDGFRa expression. The protective effects of WIN 55212-2 on PQ-induced lung injury and fibrosis were associated with an increase inM2 cells and increased expressions of IL-10, CD163, and CD206, suggesting that polarization of M2 macrophages may be involved in WIN 55212-2 protective effects on PQ-induced lung injury.
基金：
Yunnan Financial Social Security Departments Emergency Fund [116]
第一作者机构：[1]Department of Emergency, the First People’s Hospital of Yunnan Province,the Affiliated Hospital of Kunming University of Science and Technology, Xishan District, No.157 Jinbi Road Yunnan Province, Kunming City, China[3]To whom correspondence should be addressed at Department of Emergency, the First People’s Hospital of Yunnan Province,the Affiliated Hospital of Kunming University of Science and Technology, Xishan District, No.157 Jinbi Road Yunnan Province, Kunming City, China. Email: he_ quan_2009@126.com
通讯作者：
通讯机构：[1]Department of Emergency, the First People’s Hospital of Yunnan Province,the Affiliated Hospital of Kunming University of Science and Technology, Xishan District, No.157 Jinbi Road Yunnan Province, Kunming City, China[*1]Department of Emergency, the First People’s Hospital of Yunnan Province,the Affiliated Hospital of Kunming University of Science and Technology, Xishan District, No.157 Jinbi Road Yunnan Province, Kunming City, China.
推荐引用方式(GB/T 7714)：
He Quan,Zhang Wen,Zhang Jinjuan,et al.Cannabinoid Analogue WIN 55212-2 Protects Paraquat-Induced Lung Injury and Enhances Macrophage M2 Polarization[J].INFLAMMATION.2022,45(6):2256-2267.doi:10.1007/s10753-022-01688-z.
