M2 macrophages have been reported to be important in the progression of coronary artery disease (CAD). Thus,this study aims at exploring the diagnostic value of M2 macrophage-associated genes in CAD.Transcriptome profile of CAD and control samples were downloaded from GEO database. The proportion of immune cells were analysed using CIBERSORT. WGCNA was carried out to screen the relevant module associated with M2 macrophages. Differential CAD and control samples of expressed genes (DEGs) were identified by the limma R package.Functional enrichment analysis by means of the clusterProfiler R package. LASSO and RF algorithms were carried out to select signature genes. ROC curves were plotted to evaluate the diagnostic value of selected signature genes. The expressions of potential diagnostic markers were validated by RT-qPCR. The ceRNA network of diagnostic biomarkers was constructed via miRwalk and Starbase database. CMap database was used to screen candidate drugs in the treatment of CAD by targeting diagnostic biomarkers.A total of 166 M2 macrophage-associated genes were identified by WGCNA. By intersecting those genes with 879 DEGs, 53 M2 macrophage-associated DEGs were obtained in this study. By LASSO, RF, and ROC analyses, C1orf105, CCL22, CRYGB, FRK, GAP43, REG1P, CALB1 and PTPN21 were identified as potential diagnostic biomarkers. RT-qPCR showed the consistent expression patterns of diagnostic biomarkers between GEO dataset and clinical samples. Perhexiline, alimemazine and mecamylamine was found to be potential drugs in the treatment of CAD.We identified eight M2 macrophage-associated diagnostic biomarkers and candidate drugs for the CAD treatment.Copyright 2022 The Author(s).
基金：
This work was supported by National Natural Science Foundation of China (NSFC) [grant number
472 81960068]; The YunLing Scholars and Special Joint Program of Yunnan Province[grant number
473 KH-SWR-YLXZHX-2020-001]; Yunnan Key Laboratory of Innovative Application of Traditional
474 Chinese Medicine[grant number 202105AG070032]; Scientific Research Fund of Yunnan
475 Education Department[grant number 2022Y129]; the Kunming Medical University Joint Special
476 Project of Yunnan Province[grant number 202001AY070001-285]; and the Kunming Medical
477 University Joint Special Project of Yunnan Province[grant number 202101AY070001-265].
基金编号：grant number 202105AG070032grant number 2022Y129grant number 202001AY070001-285grant number 202101AY070001-265
第一作者机构：[1]Yan 'an Hospital affiliated to Kunming Medical University, Kunming, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Li Kunlin,Kong Ruize,Ma Lijing,et al.Identification of potential M2 macrophage-associated diagnostic biomarkers in coronary artery disease[J].BIOSCIENCE REPORTS.2022,42(12):doi:10.1042/BSR20221394.
APA：
Li Kunlin,Kong Ruize,Ma Lijing,Cao Yu,Li Wei...&Jiang Lihong.(2022).Identification of potential M2 macrophage-associated diagnostic biomarkers in coronary artery disease.BIOSCIENCE REPORTS,42,(12)
MLA：
Li Kunlin,et al."Identification of potential M2 macrophage-associated diagnostic biomarkers in coronary artery disease".BIOSCIENCE REPORTS 42..12(2022)
