Background: Ultraviolet (UV) exposure is the most essential etiological factor in sebaceous gland carcinoma (SGC). The abnormal expression of microRNAs (miRNAs) is also involved in SGC. However, the function of miRNAs in UV-induced SGC is still unclear. Methods: In this study, the expression levels of miR-651-5p and zinc finger E-box binding homeobox 2 (ZEB2) in SGC tissues and cells were measured by real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting. Then, the effects of rniR-651-5p on the apoptosis, migration, invasion, and epithelial-mesenchyrnal transition (EMT) of UV-induced SGC cells were determined. The interactions between miR-651-5p and ZEB2 were verified by a dual-luciferase reporter assay. An in vivo tumor growth assay was performed to assess tumorigenicity. Results: The results showed that there was abnormal expression of miR-651-5p and ZEB2 in SGC tissues and cells compared with the control tissues and cells. Overexpression of mi R-651-5p and knockdown of ZEB2 inhibited the malignant biological behaviors of SGC cells. Moreover, ZEB2 is one of the target genes of tniR-651-5p, and the expression of ZEB2 was negatively regulated by tniR-651-5p in SGC cells. Further studies showed that overexpression of miR-651-5p promoted cell apoptosis and inhibited the cell invasion and migration ability and EMT of UV-induced SGC cells by downregulating the expression of ZEB2 in vitro and in vivo. Conclusions: This study revealed that overexpression of miR-651-5p inhibited UV-induced SGC growth and metastasis by suppressing ZEB2, which may be a potential target for SGC prevention and therapy.
基金：
Science and Technology Talents and Platform Plan of Yunnan Province [2019HB050]; Yunnan Science and Technology Commission from Yunnan provincial Science and Technology Department and Kunming Medical University [2018FE001-180]; Youth Top-Notch Talent Project of Ten Thousand People Plan of Yunnan Province [YNWRQNBJ-2018-315]; Yunnan Jing Shangzhen Expert Workstation [202005AF150030]
通讯机构：[1]Kunming Univ Sci & Technol, Fac Environm Sci & Engn, 172 Jinbi Rd, Kunming 650032, Yunnan, Peoples R China[2]Kunming Univ Sci & Technol, Affiliated Hosp, Dept Ophthalmol, Peoples Hosp Yunnan Prov 1, 172 Jinbi Rd, Kunming 650032, Yunnan, Peoples R China[*1]Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Department of Ophthalmology, The Affiliated Hospital of Kunming University of Science and Technology, The First People’s Hospital of Yunnan Province, No. 172 Jinbi Road, Xishan District, Kunming 650032, China
推荐引用方式(GB/T 7714)：
Zhao Haiyan,Yang Xiaochun,Liu Juan,et al.Overexpression of miR-651-5p inhibits ultraviolet radiation-induced malignant biological behaviors of sebaceous gland carcinoma cells by targeting ZEB2[J].ANNALS OF TRANSLATIONAL MEDICINE.2022,10(9):doi:10.21037/atm-21-3897.
APA：
Zhao, Haiyan,Yang, Xiaochun,Liu, Juan,Han, Fang,Yang, Zhengrong...&Mei, Yan.(2022).Overexpression of miR-651-5p inhibits ultraviolet radiation-induced malignant biological behaviors of sebaceous gland carcinoma cells by targeting ZEB2.ANNALS OF TRANSLATIONAL MEDICINE,10,(9)
MLA：
Zhao, Haiyan,et al."Overexpression of miR-651-5p inhibits ultraviolet radiation-induced malignant biological behaviors of sebaceous gland carcinoma cells by targeting ZEB2".ANNALS OF TRANSLATIONAL MEDICINE 10..9(2022)
