Non-small cell lung cancer (NSCLC) is currently one of the malignant tumors with the highest incidence and mortality rate in China. Circular RNA hsa_circ_0000896 (circFARSA) has been reported as being an oncogene and a potential biomarker for NSCL. However, the functional role and action mechanism of circFARSA in NSCLC progression have not been fully elucidated. The present study demonstrated that circFRASA was upregulated in NSCLC tissues and cell lines, and its expression was positively correlated with poor prognosis of patients with NSCLC. Further experiments revealed that circFARSA knockdown inhibited cell proliferation, migration, and invasion in vitro experiments, but overexpression of circFARSA exhibited opposite results. Mechanistically, circFARSA facilitated the malignant phenotype of NSCLC cells by enhancing B7H3 expression through sponging miR-15a-5p. In vivo experiments, knockdown of circFARSA restricted tumor growth and metastasis. In conclusion, circFARSA served as a sponge of miR-15a-5p to promote tumorigenesis and development of NSCLC by upregulation of B7H3 expression, which provided evidence of circFARSA maybe act as a novel therapeutic target for NSCLC.
基金：
National Natural Science Foundation of China [81660012, 81970053, 81570046, 91739109]; Yunnan Provincial Clinical Medical Center Open Project [2021LCZXXF-HX09]; Research Fund for the Doctoral Program of The First People's Hospital of Yunnan Province [KHBS-2022-030]; Joint Application Foundation Project of Kunming Medical University [202201AY070001-224]; Yunnan Provincial Science and Technology Department [202201AY070001-224]; Yunnan Provincial Clinical Medical Research Center for Respiratory System Diseases-Clinical Translational Research on Respiratory Intractable Diseases [202102AA100057]; Yunnan Provincial Clinical Medical Center for Respiratory System Diseases [ZX2019-01-03]; Academician Zhong Nanshan Workstation [2019IC032-1]
第一作者机构：[1]Kunming Univ Sci & Technol, Affiliated Hosp, Peoples Hosp Yunnan Prov 1, Dept Pulm & Crit Care Med, 157 Jinbi Rd, Kunming 650032, Yunnan, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Kunming Univ Sci & Technol, Affiliated Hosp, Peoples Hosp Yunnan Prov 1, Dept Pulm & Crit Care Med, 157 Jinbi Rd, Kunming 650032, Yunnan, Peoples R China[2]Shenzhen Univ, Coll Life Sci & Oceanog, Vasc Dis Res Ctr, Shenzhen 518060, Peoples R China[*1]Department of Pulmonary and Critical Care Medicine, The First People’s Hospital of Yunnan Province. The Affiliated Hospital of Kunming University of Science and Technology, No. 157 Jinbi Road, Xishan District, Kunming, 650032, China[*2]Vascular Disease Research Center, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, Guangdong, China
推荐引用方式(GB/T 7714)：
Nie Ji,Yang Ruian,Zhou Ran,et al.Circular RNA circFARSA promotes the tumorigenesis of non-small cell lung cancer by elevating B7H3 via sponging miR-15a-5p[J].CELL CYCLE.2022,21(24):2575-2589.doi:10.1080/15384101.2022.2105087.
APA：
Nie, Ji,Yang, Ruian,Zhou, Ran,Deng, Yi,Li, Dengyuan...&Zhang, Yunhui.(2022).Circular RNA circFARSA promotes the tumorigenesis of non-small cell lung cancer by elevating B7H3 via sponging miR-15a-5p.CELL CYCLE,21,(24)
MLA：
Nie, Ji,et al."Circular RNA circFARSA promotes the tumorigenesis of non-small cell lung cancer by elevating B7H3 via sponging miR-15a-5p".CELL CYCLE 21..24(2022):2575-2589
