机构：[1]National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.[2]Department of Cellular and Molecular Pharmacology, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.[3]Department of Breast Surgery, The Third Affiliated Hospital, Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
Long non-coding RNAs (lncRNAs) are involved in cancer progression. In the present study, we analyzed the lncRNA profiles in adriamycin-resistant and -sensitive breast cancer cells and found a group of dysregulated lncRNAs in the adriamycin-resistant cells. Expression of the dysregulated lncRNAs was correlated with dysregulated mRNAs, and these were enriched in GO and KEGG pathways associated with cancer progression and chemoresistance development. Among these lncRNA-mRNA interactions, some lncRNAs may cis‑regulate neighboring protein-coding genes and be involved in chemoresistance. We then validated that the lncRNA NONHSAT028712 regulated nearby CDK2 and interfered with the cell cycle and chemoresistance. Furthermore, we identified another group of lncRNAs that trans-regulated genes by interacting with different transcription factors. For example, NONHSAT057282 and NONHSAG023333 modulated chemoresistance and most likely interacted with the transcription factors ELF1 and E2F1, respectively. In conclusion, in the present study, we report for the first time the lncRNA expression patterns in adriamycin-resistant breast cancer cells, and provide a group of novel lncRNA targets that mediate chemoresistance development in both cis- and trans-action modes.
基金：
China National Natural
Science Foundation (81572940 and 91439131 to X.M.; 31550006
to D.X.H.), the Natural Science Foundation for Distinguished
Young Scholars of Jiangsu Province (BK20140004 to X.M.),
the National High Technology Research and Development
Program (863 Program) of China (SQ2015AA020948 to
X.M.) and the Fundamental Research Funds for the Central
Universities (JUSRP51311A and JUSRP51615B to X.M. and
D.X.H.).
通讯机构：[2]Department of Cellular and Molecular Pharmacology, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.[3]Department of Breast Surgery, The Third Affiliated Hospital, Kunming Medical University, Kunming, Yunnan 650031, P.R. China.[*1]Department of Cellular and Molecular Pharmacology, School of Medicine and Pharmaceutics, Jiangnan University, Li Hu Street, Wuxi, Jiangsu 214122, P.R. China[*2]Department of Breast Surgery, The Third Affiliated Hospital, Kunming Medical University, People's West Road, Kunming, Yunnan 650031, P.R. China
推荐引用方式(GB/T 7714)：
He Dong-Xu,Zhang Guang-Yuan,Gu Xiao-Ting,et al.Genome-wide profiling of long non-coding RNA expression patterns in anthracycline-resistant breast cancer cells[J].International journal of oncology.2016,49(4):1695-1703.doi:10.3892/ijo.2016.3665.
APA：
He Dong-Xu,Zhang Guang-Yuan,Gu Xiao-Ting,Mao Ai-Qin,Lu Chun-Xiao...&Ma Xin.(2016).Genome-wide profiling of long non-coding RNA expression patterns in anthracycline-resistant breast cancer cells.International journal of oncology,49,(4)
MLA：
He Dong-Xu,et al."Genome-wide profiling of long non-coding RNA expression patterns in anthracycline-resistant breast cancer cells".International journal of oncology 49..4(2016):1695-1703
