Identification of two novel lipid metabolism-related long non-coding RNAs (SNHG17 and LINC00837) as potential signatures for osteosarcoma prognosis and precise treatment
机构：[1]Clinical Medical College of Dali University, Dali, Yunnan 671000, China[2]Department of Orthopedics, The First People’s Hospital of Dali City, Yunnan 671000, Dali, China[3]Department of Orthopedics, Kunming Medical University, Kunming, Yunnan, China[4]Department of Orthopedics, The 920th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army, Kunming, Yunnan, China[5]Department of Orthopedics, Yunnan University of Chinese Medicine, Kunming, Yunnan, China
ObjectiveDysregulated lipid metabolism enhances the development and advancement of many cancers, including osteosarcoma (OS); however, the underlying mechanisms are still largely unknown. Therefore, this investigation aimed to elucidate novel potential lipid metabolism-related long non-coding RNAs (lncRNAs) that regulate OS development and provide novel signatures for its prognosis and precise treatment.Materials and methodsThe GEO datasets (GSE12865 and GSE16091) were downloaded and analyzed using R software packages. Immunohistochemistry (IHC) was used to evaluate protein levels in OS tissues while real-time qPCR was used to measure lncRNA levels, and MTT assays were used to assess OS cell viability.ResultsTwo lipid metabolism-associated lncRNAs (LM-lncRNAs), small nucleolar RNA host gene 17 (SNHG17) and LINC00837, were identified as efficient and independent prognostic indicators for OS. In addition, further experiments confirmed that SNHG17 and LINC00837 were significantly elevated in OS tissues and cells than para-cancerous counterparts. Knockdown of SNHG17 and LINC00837 synergistically suppressed the viability of OS cells, whereas overexpression of the two lncRNAs promoted OS cell proliferation. Moreover, bioinformatics analysis was conducted to construct six novel SNHG17-microRNA-mRNA competing endogenous RNA (ceRNA) networks, and three lipid metabolism-associated genes (MIF, VDAC2, and CSNK2A2) were found to be abnormally upregulated in OS tissues, suggesting that they were potential effector genes of SNHG17.ConclusionIn summary, SNHG17 and LINC00837 were found to promote OS cell malignancy, suggesting their use as ideal biomarkers for OS prognosis and treatment.
基金：
Yunnan Traumatology and Orthopedics Clinical Medical Center [ZX20191001]; Technical Innovation Talents Training Object Project [202005AD160146]; Yunnan Orthopedics and Sports Rehabilitation Clinical Medicine Research Center [202102AA310068]
第一作者机构：[1]Clinical Medical College of Dali University, Dali, Yunnan 671000, China
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推荐引用方式(GB/T 7714)：
Tang Zhifang,Feng Hanzhen,Shu Longjun,et al.Identification of two novel lipid metabolism-related long non-coding RNAs (SNHG17 and LINC00837) as potential signatures for osteosarcoma prognosis and precise treatment[J].BMC MEDICAL GENOMICS.2023,16(1):doi:10.1186/s12920-023-01553-4.
APA：
Tang, Zhifang,Feng, Hanzhen,Shu, Longjun,Guo, Minzheng,Qi, Baochuang...&Li, Chuan.(2023).Identification of two novel lipid metabolism-related long non-coding RNAs (SNHG17 and LINC00837) as potential signatures for osteosarcoma prognosis and precise treatment.BMC MEDICAL GENOMICS,16,(1)
MLA：
Tang, Zhifang,et al."Identification of two novel lipid metabolism-related long non-coding RNAs (SNHG17 and LINC00837) as potential signatures for osteosarcoma prognosis and precise treatment".BMC MEDICAL GENOMICS 16..1(2023)
