高级检索
当前位置: 首页 > 详情页

Identification of key variants correlated with susceptibility of primary osteoporosis in the Chinese Han group

文献详情

资源类型:
Pubmed体系:
机构: [1]Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases &amp [2]Yunnan Stem Cell Translational Research Center, Kunming University, Kunming, China. [2]Human Phenome Institute, Zhangjiang Fudan International Innovation Center, Fudan University, Shanghai, China. [3]Orthopedics, Chongqing Jiulongpo District Hospital of Traditional Chinese Medicine, Chongqing, China. [4]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China. [5]Joint Surgery Department of the Second People's Hospital of Nanning City, Guangxi Zhuang Autonomous Region, Nanning, China.
出处:
ISSN:

关键词: age-dependent manner bMSCs CRB2 osteoporosis TBX3

摘要:
Primary osteoporosis is a systemic skeletal disease characterized by reduced bone mass and vulnerability to fractures. The genetics of osteoporosis in the Chinese population remain unclear, which hinders the prevention and treatment of osteoporosis in China. This study aimed to explore the susceptibility genes and the roles played by their variants in osteoporosis.Blood samples were collected from 45 osteoporosis patients and 30 healthy individuals, and genome-wide association study was performed on array data. The expression levels of the candidate gene in different genotypes were further determined by using quantitative real-time PCR. Moreover, the differentiation capacity of bone marrow mesenchymal stem cells under different genotypes from osteoporosis patients was investigated.The most significant variant rs1891632 located in the upstream (918 bp) region of CRB2, which could down-regulate the expression levels of CRB2 in genotype-tissue expression database and played an essential role in the regulation of osteoblastic and osteoclastic differentiation during skeletal development. Another significant variant rs1061657 located within the 3'UTR region of TBX3 gene. We found that the mRNA levels of TBX3 decreased in the bMSCs of old osteoporosis patients. Interestingly, osteoblast differentiation capacity and TBX3 mRNA levels were similar between the young healthy individuals carrying derived and ancestral allele of rs1061657, whereas the differentiation capacity and TBX3 mRNA levels dramatically declined in elderly patients with osteoporosis.The variant rs1061657 might affect the osteogenesis of bMSCs in an age-dependent manner and that TBX3 may be a key susceptibility gene for primary osteoporosis. In conclusion, CRB2 and TBX3 may influence the development of osteoporosis; additionally, rs1891632 and rs1061657, as the key variants first reported to be associated with primary osteoporosis, may potentially contribute to predicting the risk of osteoporosis (especially for older individuals) and may serve as therapeutic targets.© 2022 John Wiley & Sons Ltd/University College London.

基金:
语种:
PubmedID:
中科院(CAS)分区:
出版当年[2022]版:
大类 | 4 区 生物学
小类 | 4 区 遗传学
最新[2023]版:
大类 | 4 区 生物学
小类 | 4 区 遗传学
第一作者:
第一作者机构: [1]Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases &amp
共同第一作者:
通讯作者:
通讯机构: [1]Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases &amp [4]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China. [5]Joint Surgery Department of the Second People's Hospital of Nanning City, Guangxi Zhuang Autonomous Region, Nanning, China. [*1]Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases & Yunnan Stem Cell Translational Research Center, Kunming University, Kunming 650214, China. [*2]Joint Surgery Department of the Second People’s Hospital of Nanning City, Guangxi Zhuang Autonomous Region, Nanning, China. [*3]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
推荐引用方式(GB/T 7714):
APA:
MLA:

资源点击量:82478 今日访问量:0 总访问量:681 更新日期:2025-01-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 云南省第一人民医院 技术支持:重庆聚合科技有限公司 地址:云南省昆明市西山区金碧路157号