机构：[1]Department of Anesthesiology and Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.外科片麻醉手术科云南省第一人民医院[2]Dermatological Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China.[3]Medical School, Kunming University of Science and Technology, Kunming, China.[4]The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.云南省第一人民医院
Background: Patients with osteoporosis (OP) have a high risk of bone fracture. Abnormal bone mesenchymal stem cell (BMSC) differentiation is an essential process of OP development. In recent years, photobiomodulation has been shown to effectively promote BMSC proliferation. However, the mechanism by which photobiomodulation promotes BMSC proliferation is unclear. Long noncoding RNAs (lncRNAs) are essential mediators in multiple biological processes. The lncRNA maternally expressed gene 3 (MEG3) is a novel lncRNA gene and is related to cell proliferation. Studies have indicated that MEG3 serves as a promotor in BMSC proliferation.Objective: To investigate the effects and mechanisms of 800 nm light-emitting diode (LED) photobiomodulation in BMSC proliferation.Materials and methods: The BMSCs collected from mouse tibias and femurs were irradiated by 800 nm LED for 180 sec. CCK-8 assay was used to detect the cell viability. A dual-luciferase reporter assay was used to determine IncRNA MEG3 acted as a miR-217-5p sponge. We used reverse transcription-polymerase chain reaction (RT-PCR) and western blot to detect the mRNA and protein levels of MEG3, miR-217-5p, Notch2, Hes1, Hey2.Results: In the present study, we revealed that photobiomodulation (800 nm LED) could increase the mRNA level of MEG3, and protein levels of Notch2, Hes1, and Hey2. Moreover, we also identified that upregulated MEG3 could act as a miR-217-5p sponge to activate the Notch signaling pathway.Conclusions: The current study revealed the MEG3-related mechanism of photobiomodulation treatment in OP and identified potential gene therapies for OP.
基金：
National Key Research and Development Project [2018YFB0407201]; Yunnan Science and Technology Department [2019FB097]; Yunnan health training project of high level talents [D-2018005]; Yunnan Fundamental Research Key Projects [202101AS070046]; Yunnan High-level Scientific and Technological Talent Platform Plan [202105AC160064]
第一作者机构：[1]Department of Anesthesiology and Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.
共同第一作者：
通讯作者：
通讯机构：[4]The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.[*1]The First People’s Hospital of Yunnan Province The Affiliated Hospital of Kunming University of Science and Technology No. 157 Jinbi Road Kunming 650032 China[*2]Laser Medical Center The First People’s Hospital of Yunnan Province The Affiliated Hospital of Kunming University of Science and Technology No. 157 Jinbi Road Kunming 650032 China
推荐引用方式(GB/T 7714)：
Liu Na,Cao Lan,Peng Lei,et al.Photobiomodulation (800 nm Light-Emitting Diode) Treatment Promotes Bone Mesenchymal Stem Cell Proliferation Via Long Noncoding RNA MEG3-MicroRNA-217-5P Pathway[J].PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY.2023,41(1):10-16.doi:10.1089/photob.2021.0187.
APA：
Liu, Na,Cao, Lan,Peng, Lei,Lu, Weiwei,Dai, Xiaolin...&Zhu, Chongtao.(2023).Photobiomodulation (800 nm Light-Emitting Diode) Treatment Promotes Bone Mesenchymal Stem Cell Proliferation Via Long Noncoding RNA MEG3-MicroRNA-217-5P Pathway.PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY,41,(1)
MLA：
Liu, Na,et al."Photobiomodulation (800 nm Light-Emitting Diode) Treatment Promotes Bone Mesenchymal Stem Cell Proliferation Via Long Noncoding RNA MEG3-MicroRNA-217-5P Pathway".PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY 41..1(2023):10-16
