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MiR-125b and SATB1-AS1 might be shear stress-mediated therapeutic targets

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机构: [1]Department of Vascular Surgery, Affiliated Hospital of Yunnan University, Kunming 650021, Yunnan, China [2]Department of Pharmaceutical, The Third People’s Hospital of Yunnan Province, Kunming 650011, Yunnan, China [3]Department of Radiology, the First People’s Hospital of Anning, China
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关键词: Noncoding RNA expression profiles Abdominal aortic aneurysm Endovascular aneurysm repair Shear stress Molecular mechanism

摘要:
The aim of the study was to explore the potential molecular mechanism associated with shear stress on abdominal aortic aneurysm (AAA) progression. This study performed RNA sequencing on AAA patients (SQ), AAA patients after endovascular aneurysm repair (EVAR, SH), and normal controls (NC). Furthermore, we identified the differentially expressed microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNA (cirRNAs) and constructed competing endogenous RNA (ceRNA) networks. Finally, 164 differentially expressed miRNAs, 179 co-differentially expressed lncRNAs, and 440 co-differentially expressed circRNAs among the three groups were obtained. The differentially expressed miRNAs mainly enriched in 325 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Target genes associated with co-differentially expressed genes among the group of SH, SQ, and NC mainly enriched in 66 KEGG pathways. LncRNA-miRNA-mRNA interactions, including 15 lncRNAs, 63 miRNAs and 57 mRNAs, was constructed. CircRNA-miRNA-mRNA ceRNA network included 79 circRNAs, 21 miRNAs, and 49 mRNAs. Among them, KLRC2 and CSTF1, targeted by miR-125b, participated in cell-mediated immunity regulation. MiR-320-related circRNAs and SATB1-AS1 serving as the sponge of miRNAs, such as has-circ-0129245, has-circ-0138746, and has-circ-0139786, were hub genes in ceRNA network. In conclusion, AAA patients might be benefit from EVAR based on various pathways and some molecules, such as miR-125b and SATB1-AS1, related with shear stress.Copyright © 2023 Elsevier B.V. All rights reserved.

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出版当年[2023]版:
大类 | 3 区 生物学
小类 | 3 区 遗传学
最新[2023]版:
大类 | 3 区 生物学
小类 | 3 区 遗传学
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第一作者机构: [1]Department of Vascular Surgery, Affiliated Hospital of Yunnan University, Kunming 650021, Yunnan, China
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通讯机构: [1]Department of Vascular Surgery, Affiliated Hospital of Yunnan University, Kunming 650021, Yunnan, China [*1]NO. 176 Qingnian Road, Kunming 650021, Yunnan, China
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