Pelvic inflammatory disease (PID) is commonly encountered in gynecological practice. Kangfuxiaomi supposi-tory, made from the compound extract of Periplaneta Americana, is a Traditional Chinese Medicine remedy widely used for the treatment of gynecological disorders. This study aimed to preliminarily explore the therapeutic effect of Kangfuxiaomi suppository in a rat model of PID established by chemical injury and pathogen infection. The key parameters assessed were vulvar inflammation score, vaginal + uterine organ index, and serum levels of interleukin (IL)-8; tumor necrosis factor (TNF)-alpha; C-reactive protein (CRP); superoxide dismutase (SOD); and malondialdehyde (MDA). In addition, levels of IL-6, cyclooxygenase (COX)-2, and IL-2 in cervical tissues as well as that of IL-1 beta and prostaglandin E-2 (PGE2) in uterine tissues were measured. The expression levels of nuclear factor-kappa B (NF-xB) p65 and Toll-like receptor 4 (TLR4) in uterine tissues were detected by immunohisto-chemical method. After Kangfuxiaomi suppository treatment, the vulva inflammation score and histopatholog-ical score of PID rats showed a tendency to decrease. Serum IL-8, TNF-alpha, CRP, and MDA levels were reduced, while SOD levels were significantly increased. Levels of IL-6, IL-2, and COX-2 in cervical tissues were somewhat decreased, and PGE2 and IL-1 beta levels in uterine tissue were significantly decreased. Moreover, the levels of NF-xB p65 and TLR4 protein expression were also decreased. These findings demonstrated the therapeutic effect of Kangfuxiaomi suppository in PID rats. The underlying mechanism may involve enhanced antioxidant capacity and decreased secretion of proinflammatory factors via the NF-xB/TLR4 signaling pathway.