The aim of this study was to further clarify the functional mechanism of the triangular 67 (TRIM67) gene in lung cancer cells. We detected the expression of TRIM67 in lung cancer cells by RT-qPCR and Western blot, transfected si-NC, si-TRIM67, and pcDNA-ENAH into the cells. The expression of TRIM67 and ENAH was detected by Western blot and immunofluorescence localization, and CO-IP and GST pull-down experiments verified the interaction. Flow cytometry, Western blot, and transmission electron microscopy (TEM) evaluated the apoptosis and autophagy levels. TRIM67 was highly expressed in lung cancer cell lines. Knockdown of TRIM67 promoted apoptosis and autophagy of A549 and NCI-H1299 cells. TRIM67 interacted with the ENAH protein. ENAH restored the effect of knocking down TRIM67 and further inhibited apoptosis and autophagy of A549 and NCI-H1299 cells. TRIM67 inhibits apoptosis and autophagy of lung cancer cells by interacting with ENAH.
基金：
Basic Research Program of Yunnan Province (Kunming Medical Joint Project) [2019FE001]
第一作者机构：[1]First Peoples Hosp Yunnan Prov, Dept Oncol, 157 Xishan Dist, Kunming 650032, Yunnan, Peoples R China[2]Kunming Univ Sci & Technol, Affiliated Hosp, Kunming, Yunnan, Peoples R China
通讯作者：
通讯机构：[1]First Peoples Hosp Yunnan Prov, Dept Oncol, 157 Xishan Dist, Kunming 650032, Yunnan, Peoples R China[2]Kunming Univ Sci & Technol, Affiliated Hosp, Kunming, Yunnan, Peoples R China
推荐引用方式(GB/T 7714)：
Liu Rui,Miao Kun,Gao Ling,et al.TRIM67 interacts with ENAH to regulate the apoptosis and autophagy of lung cancer cells[J].GENERAL PHYSIOLOGY AND BIOPHYSICS.2024,43(2):163-173.doi:10.4149/gpb_2023037.
APA：
Liu, Rui,Miao, Kun,Gao, Ling&He, Ling.(2024).TRIM67 interacts with ENAH to regulate the apoptosis and autophagy of lung cancer cells.GENERAL PHYSIOLOGY AND BIOPHYSICS,43,(2)
MLA：
Liu, Rui,et al."TRIM67 interacts with ENAH to regulate the apoptosis and autophagy of lung cancer cells".GENERAL PHYSIOLOGY AND BIOPHYSICS 43..2(2024):163-173
