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Long noncoding RNA HOTAIR promotes breast cancer development through the lncRNA HOTAIR/miR-1/GOLPH3 axis

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资源类型：
Pubmed体系：
作者：
Zhao Jiawen[1] * ; Zhang Lei[1] * ; Zhao Yingzhu[2] * ; Wu Nan[3] * ; Zhang Xi[4] ; Guo Rong[1] ; Li Huimeng[5] ; Li Chunxiang[1] ; Zheng Kai[1] ; Liu Dequan[1] ; Tang Shicong[1] ;
机构： [1]Department of Breast Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, China. [2]Department of Breast and Thyroid Surgery, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China. [3]Department of Medical School, Yunnan College of Business Management, Kunming, China. [4]Department of Clinical Laboratory, Yunnan Cancer Hospital, Kunming, China. [5]Second Department of General Surgery, Southern Central Hospital of Yunnan Province, The First People's Hospital of Honghe State, Honghe Hospital Affiliated to Kunming Medical University, Mengzi, Honghe, China.
出处：
DOI： 10.1007/s12094-023-03197-3
ISSN：

关键词： HOTAIR miRNA-1 Breast cancer Proliferation Migration

摘要：
The lncRNA HOTAIR is frequently overexpressed in breast cancer tissues and plays an important role in the development of breast cancer. Here, we investigated the effect of the lncRNA HOTAIR on the biological behaviour of breast cancer cells and its molecular mechanism.We investigated the level of HOTAIR in breast cancer and its clinical pathological characteristics by bioinformatic methods. Then, we evaluated the effects of HOTAIR and miRNA-1 expression on the biological behaviour of breast cancer cells by qPCR, Cell Counting Kit-8 (CCK-8) assay, clonogenic assays, Transwell assay and flow cytometry for cell proliferation, invasion migration and apoptosis, and cell cycle analysis. Finally, the target genes of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis were validated by luciferase reports.The expression of HOTAIR in breast cancer tissues was significantly higher than that in normal breast tissues (P < 0.05). Silencing of HOTAIR suppressed cell proliferation, invasion and migration, promoted apoptosis and induced G1 phase block in breast cancer (P < 0.0001). We also verified that miR-1 is a target of HOTAIR and that GOLPH3 is a target of miR-1 by luciferase reporter assays (P < 0.001).The expression of HOTAIR was significantly elevated in breast cancer tissues. Reducing the expression of HOTAIR inhibited the proliferation, invasion and migration of breast cancer cells and promoted apoptosis, and the mechanism was mainly the effect of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behaviour of breast cancer cells.© 2023. The Author(s), under exclusive licence to Federación de Sociedades Españolas de Oncología (FESEO).

基金：
The present study was supported by the National Natural Science Foundation of China (grant no. 81960542 and 81960517), Science and Technology Project of Yunnan Provincial Science and Technology Department (grant no. 202001AU070053, 202001AU070093 and 202201AY070001-169), Yunnan Health Training Project of High Level Talents (grant no. H-2019075) and Beijing Science and Technology Innovation Medical Development Foundation (grant no. KC2021-JK-0044-6).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类 | 3 区 医学
小类 | 4 区 肿瘤学
最新[2023]版：
大类 | 3 区 医学
小类 | 4 区 肿瘤学
第一作者：
第一作者机构： [1]Department of Breast Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhao Jiawen,Zhang Lei,Zhao Yingzhu,et al.Long noncoding RNA HOTAIR promotes breast cancer development through the lncRNA HOTAIR/miR-1/GOLPH3 axis[J].Clinical & Translational Oncology : Official Publication Of The Federation Of Spanish Oncology Societies And Of The National Cancer Institute Of Mexico.2023,25(12):3420-3430.doi:10.1007/s12094-023-03197-3.
APA：
Zhao Jiawen,Zhang Lei,Zhao Yingzhu,Wu Nan,Zhang Xi...&Tang Shicong.(2023).Long noncoding RNA HOTAIR promotes breast cancer development through the lncRNA HOTAIR/miR-1/GOLPH3 axis.Clinical & Translational Oncology : Official Publication Of The Federation Of Spanish Oncology Societies And Of The National Cancer Institute Of Mexico,25,(12)
MLA：
Zhao Jiawen,et al."Long noncoding RNA HOTAIR promotes breast cancer development through the lncRNA HOTAIR/miR-1/GOLPH3 axis".Clinical & Translational Oncology : Official Publication Of The Federation Of Spanish Oncology Societies And Of The National Cancer Institute Of Mexico 25..12(2023):3420-3430

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