机构：[1]Department of Neurosurgery, 1st Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China昆明医科大学附属第一医院[2]Yunnan Key Laboratory of Laboratory Medicine, 1st Affiliated Hospital of Kunming Medical University, Kunming, China昆明医科大学附属第一医院[3]Department of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Health Hospital, Kunming, China[4]Department of Neurosurgery, The People’s Hospital of Chuxiong, Chuxiong, Yunnan Province, China[5]Biomedical Engineering Research Center, 6Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan Province, China. *Equal contributors.
Hypoxia is a common phenomenon in pituitary adenomas (PAs). The role and mechanism of hypoxia in the PAs remains elusive. This work aimed to explore the effect of hypoxia on PAs in vitro. PA cells GT1-1 were cultured and treated under hypoxic condition. Cell proliferation assay showed the proliferation of PA cells was increased significantly by hypoxia treatment, with a peak at 12 hours. qPCR and western blot indicated that the expression of HIF-1 alpha, ALKBH5, and Nanog were elevated by hypoxia stimuli. In conclusion, our funding demonstrated that hypoxia could increase Nanog expression through HIF-1 alpha/ALKBH5 signaling, thereby promoting the proliferation of PA cells.
基金：
Medical Academic Leader of Yunnan Province [D201618]; Health Technology Programme of Yunnan Province [2016NS057]; Innovation Team for the Effect of Neurodevelopmental Regulatory Factors in Neural Repair and Clinical Application of Yunnan Province [2018HC084]; Youth Talent Support Program of Yunnan Province
第一作者机构：[2]Yunnan Key Laboratory of Laboratory Medicine, 1st Affiliated Hospital of Kunming Medical University, Kunming, China[3]Department of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Health Hospital, Kunming, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurosurgery, 1st Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China[*1]Department of Neurosurgery, 1st Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, Kunming 650032, Yunnan Province, China.
推荐引用方式(GB/T 7714)：
Qian Yuan,Zhang Chao,Wang Wei,et al.Hypoxia promotes proliferation of pituitary adenomas by HIF-1 alpha/ALKBH5 signaling in vitro[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY.2020,13(5):1030-1034.
APA：
Qian, Yuan,Zhang, Chao,Wang, Wei,Lu, Di,Li, Junjun...&Deng, Xingli.(2020).Hypoxia promotes proliferation of pituitary adenomas by HIF-1 alpha/ALKBH5 signaling in vitro.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,13,(5)
MLA：
Qian, Yuan,et al."Hypoxia promotes proliferation of pituitary adenomas by HIF-1 alpha/ALKBH5 signaling in vitro".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 13..5(2020):1030-1034
