Efficient generation of transgene- and feeder-free induced pluripotent I stem cells from human dental mesenchymal stem cells and their chemically defined differentiation into cardiomyocytes
机构:[1]Department of Cardiology and Endodontics,the First People's Hospital of Yunnan Province,Jinbi Road No. 157,Kunming,Yunnan,China门急诊片口腔医学中心云南省第一人民医院内科片心血管内科[2]The Affiliated Hospital of Kunming University of Science and Technology,Jinbi Road No. 157,Kunming,Yunnan,China云南省第一人民医院[3]Department of Ultrasound, Dali Bai Autonomous Prefecture People's Hospital, Renminnan Road No. 35, Xiaguan, Dali, Yunnan, China[4]Department of Cardiology, First Affiliated Hospital of Kunming Medical University, Xichang Road No. 295, Kunming, Yunnan, China昆明医科大学附属第一医院
Advance in stem cell research resulted in several processes to generate induced pluripotent stem cells (iPSCs) from adult somatic cells. In our previous study, the reprogramming of iPSCs from human dental mesenchymal stem cells (MSCs) including SCAP and DPSCs, has been reported. Herein, safe iPSCs were reprogrammed from SCAP and DPSCs using non-integrating RNA virus vector, which is an RNA virus carrying no risk of altering host genome. DPSCs- and SCAP-derived iPSCs exhibited the characteristics of the classical morphology with human embryonic stem cells (hESCs) without integration of foreign genes, indicating the potential of their clinical application. Moreover, induced PSC5 showed the capacity of self renewal and differentiation into cardiac myocytes. We have achieved the differentiation of hiPSCs to cardiomyocytes lineage under serum and feeder-free conditions, using a chemically defined medium CDM3. In CDM3, hiPSCs differentiation is highly generating cardiomyocytes. The results showed this protocol produced contractile sheets of up to 97.2% TNNT2 cardiomyocytes after purification. Furthermore, derived hiPSCs differentiated to mature cells of the three embryonic germ layers in vivo and in vitro of beating cardiomyocytes. The above whole protocol enables the generation of large scale of highly pure cardiomyocytes as needed for cellular therapy. (C) 2017 Published by Elsevier Inc.
基金:
National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China [81360161]; Yunnan Provincial Science and Technology Department - Kunming Medical University Applied Basic Research Union Foundation Projects grant [2017FE468(-040)]
第一作者机构:[1]Department of Cardiology and Endodontics,the First People's Hospital of Yunnan Province,Jinbi Road No. 157,Kunming,Yunnan,China[2]The Affiliated Hospital of Kunming University of Science and Technology,Jinbi Road No. 157,Kunming,Yunnan,China
通讯作者:
推荐引用方式(GB/T 7714):
Tan Xiaobing,Dai Qingli,Guo Tao,et al.Efficient generation of transgene- and feeder-free induced pluripotent I stem cells from human dental mesenchymal stem cells and their chemically defined differentiation into cardiomyocytes[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2018,495(4):2490-2497.doi:10.1016/j.bbrc.2017.12.007.
APA:
Tan, Xiaobing,Dai, Qingli,Guo, Tao,Xu, Jingshu&Dai, Qingyuan.(2018).Efficient generation of transgene- and feeder-free induced pluripotent I stem cells from human dental mesenchymal stem cells and their chemically defined differentiation into cardiomyocytes.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495,(4)
MLA:
Tan, Xiaobing,et al."Efficient generation of transgene- and feeder-free induced pluripotent I stem cells from human dental mesenchymal stem cells and their chemically defined differentiation into cardiomyocytes".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495..4(2018):2490-2497