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Hsa_circ_0046264 up-regulated BRCA2 to suppress lung cancer through targeting hsa-miR-1245

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机构： [1]Biomedical Research Center, the Affiliated Calmette Hospital of Kunming Medical University (the First Hospital of Kunming), No. 504 Qingnian Road, Kunming 650011, Yunnan, China [2]Department of Anesthesiology, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China [3]Department of Neurosurgery, the Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), Kunming 650118, Yunnan, China [4]Department of Colorectal Cancer, the Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), Kunming 650118, Yunnan, China [5]State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China

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关键词： hsa_circ_0046264 Hsa-miR-1245 BRCA2 Lung cancer

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Objective: Lung cancer had been leading mounts of deaths worldwide. Advances in genes microarray had helped human further understand genes and identify novel circular RNAs. This study aimed at investigating the biological functions and molecular mechanisms of hsa_circ_0046264 in lung cancer which may be helpful in lung cancer early diagnosis and clinical treatment. Methods: Gene microarray data screened the differential gene of hsa_circ_0046264 and its downstream genes were found by bioinformatics analysis and verified by luciferase reporter assay. QRT-PCR and Western blot was used to detect the RNA and protein levels respectively. RNase R digestion confirmed the existences of circular RNA. Cell viability, invasion and apoptosis were determined by MTT assay, flow cytometry and DNA damage assay. Tumor formation in nude mice and immunohistochemistry proved the functions of hsa_circ_0046264 in vivo. Results: Hsa_circ_0046264 and BRCA2 were down-regulated in lung cancer tissues while miR-1245 was up-regulated. Hsa_circ_0046264 induced apoptosis but inhibited proliferation and invasion of lung cancer cells through targeting miR-1245 to up-regulate BRCA2. Hsa_circ_0046264 inhibited the tumor growth in vivo. Conclusion: Hsa_circ_0046264 was a tumor suppressor in lung cancer. Overexpression of hsa_circ_0046264 could up-regulate BRCA2 expression through down-regulating of miR-1245.

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出版当年[2018]版：

大类 | 2 区医学

小类 | 3 区呼吸系统

最新[2023]版：

大类 | 2 区医学

小类 | 2 区呼吸系统

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出版当年[2017]版：

Q2 RESPIRATORY SYSTEM

最新[2023]版：

Q1 RESPIRATORY SYSTEM

影响因子： 4.7 最新[2023版] 5.3 最新五年平均 3.751 出版当年[2017版] 4.113 出版当年五年平均 3.841 出版前一年[2016版] 3.829 出版后一年[2018版]

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第一作者机构： [1]Biomedical Research Center, the Affiliated Calmette Hospital of Kunming Medical University (the First Hospital of Kunming), No. 504 Qingnian Road, Kunming 650011, Yunnan, China

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