Intracellular calcium concentration of corpus cavernosum smooth muscle cells is decreased by the overexpression of PnNOS gene in adipose tissue-derived stem cells
机构：[1]Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China中山大学附属第三医院[2]School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou Guangdong, China[3]Center of Reproductive Medicine, The First People’s Hospital of Yunnan Province, Kunming, China门急诊片生殖医学科云南省第一人民医院[4]Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China华中科技大学同济医学院附属同济医院[5]Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou,China中山大学附属第三医院
The study investigated the effects of adipose tissue-derived stem cells (ADSCs) modified with penile neuronal nitric oxide synthase (PnNOS) gene on intracellular calcium concentration in rat corpus cavernosum smooth muscle cells (CCSMCs). ADSCs and CCSMCs of Sprague-Dawley (SD) rats were isolated and cultured in vitro respectively. The rat PnNOS gene was transferred into the ADSCs mediated by a recombinant adenovirus vector. The expression of the PnNOS gene was detected. At the same time, the concentration of nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) was assayed. After coculturing with the CCSMCs of SD rats, which were isolated and expanded ex vivo, the cGMP and NO levels of ADSCs and CCSMCs were measured. Intracellular calcium concentration ([Ca2+](i)) in rat CCSMCs was measured with Fluo-3/AM by flow cytometer after cocultured with ADSCs overexpressing PnNOS gene. The mRNA and protein expression of PnNOS gene mediated by recombinant adenovirus vector significantly overexpressed and lasted at least 2weeks. Meanwhile, the concentration of NO and cGMP in ADSCs was greatly increased. The concentration of cGMP was significantly increased, and [Ca2+](i) was obviously decreased in CCSMCs compared with the control groups (P<0.05) after cocultured with ADSCs for 3days. These findings demonstrated that ADSCs overexpressing PnNOS gene might decrease [Ca2+]i in CCSMCs by up-regulating NO-cGMP signalling pathway.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81370705, 81070487, 81370702]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2013M542191]; Natural Science Foundation of Guangdong Province, ChinaNational Natural Science Foundation of Guangdong Province [S2012010009140]
第一作者机构：[1]Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China[2]School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou Guangdong, China[*1]Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600 Tianhe Road, Guangzhou 510630, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China[2]School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou Guangdong, China[3]Center of Reproductive Medicine, The First People’s Hospital of Yunnan Province, Kunming, China[4]Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China[5]Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou,China[*1]Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600 Tianhe Road, Guangzhou 510630, China[*2]Center of Reproductive Medicine, the First People’s Hospital of Yunnan Province, Kunming 650032, P. R. China[*3]Department of Urology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, P. R. China
推荐引用方式(GB/T 7714)：
Chen J.,Hu Z.,Zhuan L.,et al.Intracellular calcium concentration of corpus cavernosum smooth muscle cells is decreased by the overexpression of PnNOS gene in adipose tissue-derived stem cells[J].ANDROLOGIA.2015,47(6):711-719.doi:10.1111/and.12323.
APA：
Chen, J.,Hu, Z.,Zhuan, L.,Xiao, H.,Zhang, Y.&Yang, J..(2015).Intracellular calcium concentration of corpus cavernosum smooth muscle cells is decreased by the overexpression of PnNOS gene in adipose tissue-derived stem cells.ANDROLOGIA,47,(6)
MLA：
Chen, J.,et al."Intracellular calcium concentration of corpus cavernosum smooth muscle cells is decreased by the overexpression of PnNOS gene in adipose tissue-derived stem cells".ANDROLOGIA 47..6(2015):711-719
