机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of The Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China[2]University of The Chinese Academy of Sciences, Beijing, China[3]First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China昆明医科大学附属第一医院[4]Department of Biochemistry, Kunming Medical University, Kunming, Yunnan, China[5]Cancer Hospital, Kunming Medical University, Kunming, Yunnan, China
Growing evidence suggests that YAP/TAZ are mediators of the Hippo pathway and promote breast cancer. However, the roles of YAP/TAZ transcription factor partners TEADs in breast cancer remain unclear. Here we found that TEAD4 was expressed in breast cancer cell lines, especially in triple negative breast cancers (TNBC) cell lines. TEAD4 binds to KLF5. Knockdown of either TEAD4 or KLF5 in HCC1937 and HCC1806 cells induced the expression of CDK inhibitor p27. Depletion of either TEAD4 or KLF5 activated the p27 gene promoter and increased the p27 mRNA levels. Depletion of p27 partially prevents growth inhibition caused by TEAD4 and KLF5 knockdown. TEAD4 overexpression stimulated proliferation in vitro and tumor growth in mice, while stable knockdown of TEAD4 inhibited proliferation in vitro and tumor growth in mice. Thus TEAD4 and KLF5, in collaboration, promoted TNBC cell proliferation and tumor growth in part by inhibiting p27 gene transcription. TEAD4 is a potential target and biomarker for the development of novel therapeutics for breast cancer.
基金：
Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences; Stem Cell and Regenerative Medicine Research [XDA01040406]; National Nature Science Foundation of ChinaNational Natural Science Foundation of China [81460401, 81325016, U1132605, 81120108019]; Department of Science and Technology of Yunnan Province-Kunming Medical University [4FB022]
第一作者机构：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of The Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China[2]University of The Chinese Academy of Sciences, Beijing, China[3]First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Chunyan,Nie Zhi,Zhou Zhongmei,et al.The interplay between TEAD4 and KLF5 promotes breast cancer partially through inhibiting the transcription of p27(Kip1)[J].ONCOTARGET.2015,6(19):17685-17697.doi:10.18632/oncotarget.3779.
APA：
Wang, Chunyan,Nie, Zhi,Zhou, Zhongmei,Zhang, Hailin,Liu, Rong...&Chen, Ceshi.(2015).The interplay between TEAD4 and KLF5 promotes breast cancer partially through inhibiting the transcription of p27(Kip1).ONCOTARGET,6,(19)
MLA：
Wang, Chunyan,et al."The interplay between TEAD4 and KLF5 promotes breast cancer partially through inhibiting the transcription of p27(Kip1)".ONCOTARGET 6..19(2015):17685-17697
医学