机构：[1]Department of Head and Neck, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming,[2]Department of Head and Neck, The First Affiliated Hospital of Kunming Medical University, Kunming,昆明医科大学附属第一医院[3]Medical Faculty, Kunming University of Science and Technology, Kunming,[4]The First People’s Hospital of Yunnan Province (The Affiliated Hospital of Kunming University of Science and Technology), Kunming,云南省第一人民医院[5]Kunming Institute of Precious Metals, Kunming,[6]Department of cardiothoracic surgery, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming,[7]Institute of Oncology, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming,[8]Clinical skills training center of Kunming Medical University, Kunming,[9]Department of Anesthesiology, Yan An Hospital, Kunming, Yunnan, The People’s Republic of China
Gold nanorods (AuNRs) have been used in plasmonic photothermal therapy (PPTT), which is thought to be more efficient and selective than conventional photothermal therapy. The efficiency and safety of PPTT can be improved by functionally modifying the gold nanorods with proteins or biomolecules. In this study, AuNRs were modified with anti-epidermal growth factor receptor (EGFR) monoclonal antibody (mAb), and the apoptotic potential of EGFRmAb-AuNR was assessed in Hep-2 cells in vitro and in vivo. The EGFRmAb modification had no obvious influence on the original optical property of the AuNRs, but it significantly increased the entry of AuNRs into Hep-2 cells. EGFRmAb-AuNRs, with appropriate laser irradiation, resulted in higher Hep-2 cells apoptosis than AuNRs did alone, in vitro, and was accompanied by alteration of reactive oxygen species (ROS) production, Ca2+ release, change in mitochondrial membrane potential (Delta Psi m), cytochrome c (Cyt-c) release, active caspase-3 expression, and level of B-cell lymphoma 2 (Bcl-2) and B-cell lymphoma 2 protein-associated X protein (Bax). EGFRmAb-AuNR-mediated apoptosis in Hep-2 cells was also observed in vivo and had an inhibitive effect on growth of Hep-2 tumor xenografts. Our data suggest that the EGFRmAb modification improves AuNR-mediated apoptosis and may have the potential to be used clinically.
基金：
National Natural Science Foundation of China (81160324) and Kunming University of Science and Technology (KKZ3201360025).
第一作者机构：[1]Department of Head and Neck, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming,[2]Department of Head and Neck, The First Affiliated Hospital of Kunming Medical University, Kunming,
共同第一作者：
通讯作者：
通讯机构：[2]Department of Head and Neck, The First Affiliated Hospital of Kunming Medical University, Kunming,[*1]Department of Head and Neck, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, People’s Republic of China
推荐引用方式(GB/T 7714)：
Zhang Shiwen,Li Yunlong,He Xiaoguang,et al.Photothermolysis mediated by gold nanorods modified with EGFR monoclonal antibody induces Hep-2 cells apoptosis in vitro and in vivo[J].INTERNATIONAL JOURNAL OF NANOMEDICINE.2014,9:1931-1946.doi:10.2147/IJN.S59870.
APA：
Zhang, Shiwen,Li, Yunlong,He, Xiaoguang,Dong, Shouan,Huang, Yunchao...&Wang, Yuanling.(2014).Photothermolysis mediated by gold nanorods modified with EGFR monoclonal antibody induces Hep-2 cells apoptosis in vitro and in vivo.INTERNATIONAL JOURNAL OF NANOMEDICINE,9,
MLA：
Zhang, Shiwen,et al."Photothermolysis mediated by gold nanorods modified with EGFR monoclonal antibody induces Hep-2 cells apoptosis in vitro and in vivo".INTERNATIONAL JOURNAL OF NANOMEDICINE 9.(2014):1931-1946
医学