机构：[a]Oncology Department, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China昆明医科大学附属第一医院[b]Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China[c]The Affiliated Zhongda Hospital of Southeast University, Nanjing 21009, Jiangsu, China[d]Nanjing Maternal and Child Health Hospital, 123# Tianfeixiang, Baixia District, Nanjing 210004, Jiangsu, China
A novel lumbricin-like antimicrobial peptide named lumbricin-PG was isolated from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen), using a procedure of one step Sephadex G-50 gel filtration and one step C(8) reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEMPAEGGHIEP by Edman degradation combined with cDNA cloning and mass spectrometry analysis. The cDNA encoding lumbricin-PG was cloned by cDNA library screening. The predicted protein from the cDNA sequence was composed of 73 amino acid residues including a mature lumbricin-PG and predicted signal peptide. It showed similarity with lumbricin antimicrobial peptide from the earthworm, Lumbricus rubellus by BLAST search. Purified lumbricin-PG exerted potential antimicrobial activities against bacteria and fungi; it showed weak hemolysis activity against human and rabbit red cells. (C) 2011 Elsevier Inc. All rights reserved.
第一作者机构：[a]Oncology Department, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China[b]Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China
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推荐引用方式(GB/T 7714)：
Wenliang Li,Sisi Li,Jian Zhong,et al.A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen)[J].PEPTIDES.2011,32(6):1146-1150.doi:10.1016/j.peptides.2011.04.015.
APA：
Wenliang Li,Sisi Li,Jian Zhong,Zhu Zhu,Jingze Liu&Wenhong Wang.(2011).A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen).PEPTIDES,32,(6)
MLA：
Wenliang Li,et al."A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen)".PEPTIDES 32..6(2011):1146-1150
