Adenosine A2A Receptor Agonist PSB-0777 Modulates Synaptic Proteins and AMPA Receptor Expression in A Dose- and Time-Dependent Manner in Rat Primary Cortical Neurons.
机构：[1]Yunnan University, School of Medicine, 2 Cuihu North Road, Kunming, Yunnan, P. R. China, 650091[2]Yunnan University, School of Life Sciences, Center for Life Sciences, 2 Cuihu North Road, Kunming, Yunnan, P. R. China, 650091[3]First Affliated Hospital of Kunming Medical University, Department of Medical Examination, Xichang Road, Kunming, Yunnan, P. R. China, 650032
Regulating synaptic formation and transmission is critical for the physiology and pathology of psychiatric disorders. The adenosine A2A receptor subtype has attracted widespread attention as a key regulator of neuropsychiatric activity, neuroprotection and injury. In this study, we systematically investigated the regulatory effects of a novel A2A receptor agonist, PSB-0777, on the expression of synaptic proteins and AMPA receptors at the cellular level in a time- and dose-dependent manner. After 30 minutes of high-dose PSB-0777 stimulation, the expression of Syn-1, PSD95, and AMPA receptors and the number of synapses were rapidly and significantly increased in rat primary cortical neurons compared with the control. Sustained elevation was found in the low and medium-dose groups after 24 hours and 3 days of treatment. In contrast, after stimulation with PSB-0777 for 3 consecutive days, the expression of Syn-1 was decreased, and PSD95, AMPA receptors and the number of synapses were no longer increased in the high-dose group. Our study focuses on the detailed and systematic regulation of synaptic proteins and AMPA receptors by an A2A receptor agonist, PSB-0777, which may result in both beneficial and detrimental effects on neurotransmission and neuroprotection and may contribute to the pathophysiology of psychiatric disorders related to A2A receptors. These experimental data may contribute to understanding of the mechanisms for neuroprotective and therapeutic effect of A2A receptor agonists.
基金：
The authors gratefully acknowledge the support of the National Natural Science
Foundation of China (Grant No. 81860499) and the Academic New Artist of
Distinction for Doctoral Post Graduate in Yunnan Province (C176230200).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区医学
小类|4 区药学
最新[2023]版：
大类|4 区医学
小类|4 区药学
第一作者：
第一作者机构：[1]Yunnan University, School of Medicine, 2 Cuihu North Road, Kunming, Yunnan, P. R. China, 650091
共同第一作者：
通讯作者：
通讯机构：[1]Yunnan University, School of Medicine, 2 Cuihu North Road, Kunming, Yunnan, P. R. China, 650091[*1]Yunnan University, School of Medicine, 2 Cuihu North Road, Kunming, Yunnan, P. R.China, 650091
推荐引用方式(GB/T 7714)：
Luo Shaolei,Hou Yangyang,Zhang Yaping,et al.Adenosine A2A Receptor Agonist PSB-0777 Modulates Synaptic Proteins and AMPA Receptor Expression in A Dose- and Time-Dependent Manner in Rat Primary Cortical Neurons.[J].Biological & pharmaceutical bulletin.2020,43(8):1159-1171.doi:10.1248/bpb.b19-01007.
APA：
Luo Shaolei,Hou Yangyang,Zhang Yaping,Ma Tengfei,Shao Wenping&Xiao Chunjie.(2020).Adenosine A2A Receptor Agonist PSB-0777 Modulates Synaptic Proteins and AMPA Receptor Expression in A Dose- and Time-Dependent Manner in Rat Primary Cortical Neurons..Biological & pharmaceutical bulletin,43,(8)
MLA：
Luo Shaolei,et al."Adenosine A2A Receptor Agonist PSB-0777 Modulates Synaptic Proteins and AMPA Receptor Expression in A Dose- and Time-Dependent Manner in Rat Primary Cortical Neurons.".Biological & pharmaceutical bulletin 43..8(2020):1159-1171
