机构：[1]Technology Transfer Center, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China[2]Department of Physiology, Kunming Medical University, Kunming, China[3]Department of Respiratory Medicine, Affiliated Hospital of Jining Medical University, Jining, China[4]Department of Neurology, Affiliated Hospital of Jining Medical University, Jining, China[5]Department of Thoracic Surgery, The First People’s Hospital of Honghe State, Kunming, Yunnan, China[6]Biomedical Engineering Research Center, Kunming Medical University, Kunming, China[7]Department of Plastic Surgery, The Second Affiliated Hospital of Kunming Medical Univesity, Kunming, China
Circular RNA (circRNA), a novel type of non-coding RNA that consists of a circular loop, has been demonstrated to act as a "sponge" for microRNAs (miRNAs). However, the role of circRNAs in keloid remains unknown. In this study, we investigated circRNA expression profiles in keloid to identify potential diagnostic and therapeutic circRNAs. We performed a circRNA microarray assay to determine circRNA expression in keloid and paired normal skin tissues. Quantitative reverse transcription polymerase chain reaction was used to evaluate the expression levels of candidate circRNAs. The most significantly over-expressed circRNA was used to predict putative miRNA targets and the binding sites of miRNAs with this circRNA. Finally, we constructed a circRNA-miRNA interaction network and carried out gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. We found 52 significantly upregulated and 24 downregulated circRNAs in keloid compared with normal skin tissue. We confirmed that hsa_circ_0057452, hsa_circ_0007482, hsa_circ_0020792, hsa_circ_0057342, and hsa_circ_0043688 were significantly upregulated in keloid tissues. Analysis of the circRNA-miRNA interaction network revealed that circRNAs could interact with miRNAs, including miRNA-29a, miRNA-23a-5p and miRNA-1976. GO and KEGG analyses indicated that these target genes were involved in biological functions and signaling pathways that may play vital roles in the pathogenesis of keloid. This study revealed that circRNAs are potentially implicated in the development of keloid and could serve as novel diagnostic and therapeutic targets.
基金：
Supported by grants from the National Natural
Science Foundation of China (NSFC; Grant No. 81960354 and
81560502), the National Natural Science Foundation of Yunnan Province
(Grant No.2017FB116 and 2018FE001(-235)), the Talent Project
of Yunnan Province (2019HB024) and 100 Talents Program of Kunming
Medical University (Lechun Lyu).
第一作者机构：[1]Technology Transfer Center, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming 650500, Yunnan, China[2]Department of Physiology, Kunming Medical University, Kunming, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Shi Jingpei,Yao Shuluan,Chen Pan,et al.The integrative regulatory network of circRNA and microRNA in keloid scarring[J].MOLECULAR BIOLOGY REPORTS.2020,47(1):201-209.doi:10.1007/s11033-019-05120-y.
APA：
Shi, Jingpei,Yao, Shuluan,Chen, Pan,Yang, Yuan,Qian, Mengjiao...&Lu, Di.(2020).The integrative regulatory network of circRNA and microRNA in keloid scarring.MOLECULAR BIOLOGY REPORTS,47,(1)
MLA：
Shi, Jingpei,et al."The integrative regulatory network of circRNA and microRNA in keloid scarring".MOLECULAR BIOLOGY REPORTS 47..1(2020):201-209
