机构：[1]Department of Clinical Laboratory, The Affiliated Hospital of KMUST, Medical Faculty, Kunming University of Science and Technology, Kunming, Yunnan 650032, P.R. China
Cancer stem cells are reportedly associated with drug resistance in glioma, but there are conflicting findings on the effects of cancer stem cells on drug resistance. The aim of the present study was to identify the underlying mechanisms of drug resistance in rat C6 glioma cells, through the use of Transwell invasion assays, flow cytometric and western blot analyses as well as immunohistochemical staining. The results revealed that acquisition of drug resistance by C6 cells enhanced migration ability in vivo and in vitro. Notably, drug resistance did not depend on the cancer stem cells of C6 cells, but on the increase of a side population phenotype. Blockade of the ABC transporter could increase sensitivity to temozolomide and temozolomide-induced apoptosis in C6 cells. Collectively, these data indicated that drug resistance of C6 cells was mediated by the side population phenotype rather than by cancer stem cells.
基金：
The present study was supported by the National Natural
Science Foundation of China (no. 81760503 to XY), the
National Natural Science Foundation of China (no. 81760660
to JL), the Yunnan Applied Basic Research Project [grant
no. 2018FE001(‑318)], the Yunnan Applied Basic Research
Project [grant no. 2018FE001(‑123)] and the Yunnan Health
Science and Technology Plan Projects (grant no. 2016NS207).
第一作者机构：[1]Department of Clinical Laboratory, The Affiliated Hospital of KMUST, Medical Faculty, Kunming University of Science and Technology, Kunming, Yunnan 650032, P.R. China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, The Affiliated Hospital of KMUST, Medical Faculty, Kunming University of Science and Technology, Kunming, Yunnan 650032, P.R. China[*1]Department of Clinical Laboratory, The Affiliated Hospital of KMUST, Medical Faculty, Kunming University of Science and Technology, 157 Jinbi Road, Xishan, Kunming, Yunnan 650032, P.R. China
推荐引用方式(GB/T 7714)：
Xu Ya,Yang Xiangcai,Mei Shuting,et al.Acquisition of temozolomide resistance by the rat C6 glioma cell line increases cell migration and side population phenotype[J].ONCOLOGY REPORTS.2019,42(6):2355-2362.doi:10.3892/or.2019.7350.
APA：
Xu, Ya,Yang, Xiangcai,Mei, Shuting,Sun, Yi&Li, Jiejing.(2019).Acquisition of temozolomide resistance by the rat C6 glioma cell line increases cell migration and side population phenotype.ONCOLOGY REPORTS,42,(6)
MLA：
Xu, Ya,et al."Acquisition of temozolomide resistance by the rat C6 glioma cell line increases cell migration and side population phenotype".ONCOLOGY REPORTS 42..6(2019):2355-2362
