Novel Histidine Kinase Gene HisK2301 from Rhodosporidium kratochvilovae Contributes to Cold Adaption by Promoting Biosynthesis of Polyunsaturated Fatty Acids and Glycerol
机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, PR China[2]Genetic Diagnosis Center, Yunnan Provincial Key Laboratory for Birth Defects and Genetic Diseases, The First People’s Hospital of Yunnan Province, Kunming, Yunnan 650032, PR China门急诊片医学遗传科云南省第一人民医院
Hybrid histidine kinase (HHKs) are widespread in fungi, but their roles in the regulation of fungal adaptation to environmental stresses remain largely unclear. To elucidate this, we cloned HisK2301 from Rhodosporidium kratochvilovae strain YM25235, characterized HisK2301 as a novel HHK, and further investigated the role of HisK2301 by overexpressing it in YM25235. Our results revealed that HisK2301 can promote adaptation of YM25235 to cold, osmotic, and salt stresses. During cold stress, HisK2301 significantly enhanced the biosynthesis of polyunsaturated fatty acids (PUFA) and intracellular glycerol. HisK2301 also augmented the expression levels of Delta(12)/Delta(15) fatty acid desaturase (RKDI2) and glycerol-3-phosphate dehydrogenasel (GPM), which are responsible for PUFA and glycerol biosynthesis, respectively. To conclude, our findings give the first insight into the defense and mechanisms of HisK2301 in fungi against cold stress and suggest the potential use of the novel cold-adapted HHK HisK2301 in industrial processes, such as large-scale production of PUFA.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31660454, 31160016]; Project of Medical Academic Leader of Yunnan Province [D-201643]
第一作者机构：[1]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, PR China[2]Genetic Diagnosis Center, Yunnan Provincial Key Laboratory for Birth Defects and Genetic Diseases, The First People’s Hospital of Yunnan Province, Kunming, Yunnan 650032, PR China
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推荐引用方式(GB/T 7714)：
He Jing,Cui Zhicheng,Ji Xiuling,et al.Novel Histidine Kinase Gene HisK2301 from Rhodosporidium kratochvilovae Contributes to Cold Adaption by Promoting Biosynthesis of Polyunsaturated Fatty Acids and Glycerol[J].JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.2019,67(2):653-660.doi:10.1021/acs.jafc.8b04859.
APA：
He, Jing,Cui, Zhicheng,Ji, Xiuling,Luo, Yiyong,Wei, Yunlin&Zhang, Qi.(2019).Novel Histidine Kinase Gene HisK2301 from Rhodosporidium kratochvilovae Contributes to Cold Adaption by Promoting Biosynthesis of Polyunsaturated Fatty Acids and Glycerol.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67,(2)
MLA：
He, Jing,et al."Novel Histidine Kinase Gene HisK2301 from Rhodosporidium kratochvilovae Contributes to Cold Adaption by Promoting Biosynthesis of Polyunsaturated Fatty Acids and Glycerol".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67..2(2019):653-660
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