机构：[1]College of Life Science and Technology, Kunming University of Science and Technology, Kunming, China[2]Medical Faculty, Kunming University of Science and Technology, Kunming, China[3]The Emergency Intensive Care Unit, The First People’s Hospital of Yunnan Province, Kunming, China外科片重症医学科云南省第一人民医院
Sepsis is a serious public health issue and the leading cause of death in critically ill patients in intensive care units. Thioredoxin-1 (Trx-1) is a protein of regulating redox, as well as a modulator of inflammation and apoptosis. Our previous study reported that Trx-1 decreased endoplasmic reticulum-mediated inflammation involved in lung in a model of experimental sepsis. However, its effect on mitochondrial-mediated apoptosis in spleen has not been reported. We studied whether Trx-1 could prevent spleen cells apoptosis in sepsis. In the present study, we showed that the apoptosis in spleen was decreased in sepsis induced by cecal ligation and puncture (CLP) in Trx-1 overexpression transgenic (Tg) mice compared with wild-type mice. Colony forming units in the peritoneal cavity and the level of procalcitonin in plasma were significantly decreased in Trx-1 Tg mice 12h after CLP. The expressions of c-jun-N-terminal kinase, Bax, caspase-9, and caspase-3 were increased in spleen, which were suppressed in Trx-1 Tg mice. However, the decreased Bcl-2 expression in sepsis was recovered in Trx-1 Tg mice. Our results suggest that overexpression of Trx-1 provides protection against sepsis through suppressing mitochondria-induced apoptosis pathway in spleen. This study may provide a new target for clinical intervention, as well potential strategies for treatment of sepsis.
基金：
This study was supported by National Natural Science Foundation of China (Nos.
81160162, U1202227, 81660316), Program for Innovative Research Team (in
Science and Technology) in University of Yunnan Province (2014), and a grant
from the fund of Department of Science and Technology of Yunnan ProvinceKunming Medical University joint/union (No. 2016FB124).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区医学
小类|2 区外科3 区危重病医学3 区血液学3 区外周血管病
最新[2023]版：
大类|3 区医学
小类|3 区血液学3 区外周血管病3 区外科4 区危重病医学
JCR分区：
出版当年[2016]版：
Q1SURGERYQ2CRITICAL CARE MEDICINEQ2PERIPHERAL VASCULAR DISEASEQ2HEMATOLOGY
最新[2023]版：
Q1SURGERYQ2CRITICAL CARE MEDICINEQ2HEMATOLOGYQ2PERIPHERAL VASCULAR DISEASE
第一作者机构：[1]College of Life Science and Technology, Kunming University of Science and Technology, Kunming, China[2]Medical Faculty, Kunming University of Science and Technology, Kunming, China[3]The Emergency Intensive Care Unit, The First People’s Hospital of Yunnan Province, Kunming, China
通讯作者：
通讯机构：[2]Medical Faculty, Kunming University of Science and Technology, Kunming, China[*1]Medical Faculty, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming 650500, China
推荐引用方式(GB/T 7714)：
Chen Guobing,Li Xiang,Huang Mengbing,et al.The Role of Thioredoxin-1 in Suppression Sepsis Through Inhibiting Mitochondrial-Induced Apoptosis in Spleen[J].SHOCK.2017,47(6):753-758.doi:10.1097/SHK.0000000000000789.
APA：
Chen, Guobing,Li, Xiang,Huang, Mengbing,Zhou, Xiaoshuang,Li, Ye...&Bai, Jie.(2017).The Role of Thioredoxin-1 in Suppression Sepsis Through Inhibiting Mitochondrial-Induced Apoptosis in Spleen.SHOCK,47,(6)
MLA：
Chen, Guobing,et al."The Role of Thioredoxin-1 in Suppression Sepsis Through Inhibiting Mitochondrial-Induced Apoptosis in Spleen".SHOCK 47..6(2017):753-758
