机构：[1]Institute of Clinical and Basic Medical Sciences, Center of Clinical Molecular Biology, Key Laboratory for Birth Defects and Genetic Diseases, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, China云南省第一人民医院[2]State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China[3]Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, Toronto, Canada
To investigate the role hepatitis Be antigen (HBeAg) plays in the evolution of hepatitis B virus (HBV), we sequenced the basic core promoter (BCP) and precore (preC) regions of 348 clones total from ten HBV Chinese patients. Eleven mutations were more frequent in HBeAg-negative patients than in HBeAg-positive patients. Further, the sequencing of dozens of variants was found to be necessary to obtain mutation profiles. Phylogenetic and median-joining network analyses suggested that variants from each patient had a single common ancestor (monophyly). Higher haplotype and nucleotide diversities were identified in HBeAg-negative"patients. Analysis of dNIdS suggested that viruses experiencing a stronger immune response had lower haplotype diversity. Because HBeAg seroconversion was associated with viral diversity it served as an indicator of HBV evolution. Significantly, this study indicated a larger sampling of variants from each patient was required to understand effectively the properties of HBV. (C) 2013 Elsevier B.V. All rights reserved.
基金：
Natural Science Foundation ofYunnan Province (Grant no. 200300172), in part by special fund ofScience and Technology Department of Yunnan Province in asso-ciation with Kunming Medical College (2012FB097), in part byFoundation of Yunnan Provincial Bureau of Health (2010NS004),in part by National Natural Science Foundation of China (No.81160352), in part by a Visiting Professorship for Senior Interna-tional Scientists from the Chinese Academy of Sciences, and in partby Discovery Grant 3148 from the Natural Sciences and EngineeringResearch Council of Canada.
第一作者机构：[1]Institute of Clinical and Basic Medical Sciences, Center of Clinical Molecular Biology, Key Laboratory for Birth Defects and Genetic Diseases, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, China
共同第一作者：
通讯作者：
通讯机构：[1]Institute of Clinical and Basic Medical Sciences, Center of Clinical Molecular Biology, Key Laboratory for Birth Defects and Genetic Diseases, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, China[*1]Institute of Clinical and Basic Medical Sciences, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University ofScience and Technology, Kunming, China
推荐引用方式(GB/T 7714)：
He Jun-Dong,Gao Jian-Mei,Shen Tao,et al.Evolutionary perspective on hepatitis B virus with an expanded sampling strategy[J].VIRUS RESEARCH.2013,178(2):525-529.doi:10.1016/j.virusres.2013.09.032.
APA：
He, Jun-Dong,Gao, Jian-Mei,Shen, Tao,Murphy, Robert W.&Yan, Xin-Min.(2013).Evolutionary perspective on hepatitis B virus with an expanded sampling strategy.VIRUS RESEARCH,178,(2)
MLA：
He, Jun-Dong,et al."Evolutionary perspective on hepatitis B virus with an expanded sampling strategy".VIRUS RESEARCH 178..2(2013):525-529
