Hepatitis B virus (HBV) infection is highly prevalent in China. To identify the genotypes of HBV in the southern Yunnan Province of China, full-length HBV genomes were extracted from 1 Dai and 4 Hani HBV carriers and linked with the pMD T-18 vector. For each patient, 3-10 clones were sequenced directly and a consensus sequence was created. Genotypic and serotypic analysis revealed 4 HBV/B (2 B2 with adw2 and 2 new subgenotypes with ayw1) and 1 HBV/C (C1 with adrq+) genotypes. The divergences of the entire genome sequences of the new subgenotype were 0-0.9% and 2.99-6.48% between other known HBV/B. Divergences in other coding regions revealed that it was more similar to B3 and B4 in the precore/core gene (2.02 and 2.09%, respectively), and similar to B3 and B5 in the preS1/S2/S gene (2.24 and 2.78%, respectively). Phylogenetic trees using the precore/core and X genes both revealed a new clad separating from the major trunk of genotype B with a 99% bootstrap value. These results show that the 2 consensus isolates are a mosaic of B3 B5, which we designated to subgenotype B6. Considering the geographical distances, the relationship between B6 and other HBV/B subgenotypes (B3-B5) and HBV evolution needs to be further studied. Copyright (C) 2009 S. Karger AG, Basel
基金：
Natural Science Foundation
of Yunnan Province (grant No. 200300172), P.R. China, and part
by the Hospital Science Foundation of the First People’s Hospital
of Yunnan Province (2004, 2007).
第一作者机构：[1]First Peoples Hosp Yunnan Prov, Inst Basic Med, Kunming 650032, Yunnan Province, Peoples R China[2]Ctr Clin Mol Biol, Kunming, Peoples R China[3]Kunhua Affiliated Hosp, Kunming Med Coll, Kunming, Peoples R China
通讯作者：
通讯机构：[1]First Peoples Hosp Yunnan Prov, Inst Basic Med, Kunming 650032, Yunnan Province, Peoples R China[2]Ctr Clin Mol Biol, Kunming, Peoples R China[3]Kunhua Affiliated Hosp, Kunming Med Coll, Kunming, Peoples R China[*1]Institute of Basic Medicine of the First People’s Hospital of Yunnan Province No.157 Jin Bi Road Kunming 650032, Yunnan Province (PR China)
推荐引用方式(GB/T 7714)：
Shen Tao,Gao Jian-mei,Zou Yun-Lian,et al.Novel Hepatitis B Virus Subgenotype in the Southern Yunnan Province of China[J].INTERVIROLOGY.2009,52(6):340-346.doi:10.1159/000252912.
APA：
Shen, Tao,Gao, Jian-mei,Zou, Yun-Lian,Dong, Hong&Yan, Xin-Min.(2009).Novel Hepatitis B Virus Subgenotype in the Southern Yunnan Province of China.INTERVIROLOGY,52,(6)
MLA：
Shen, Tao,et al."Novel Hepatitis B Virus Subgenotype in the Southern Yunnan Province of China".INTERVIROLOGY 52..6(2009):340-346
