According to the gray value in CT image sequence of NSCLC, the visualization of CT images can facilitated surgeries to analyze and judge detailed characteristics inside the tumor. Through the color distribution of the heat map, it can identify a representative position for tumor puncture biopsy. CT images sequence is reconstructed three-dimensional model, including the skin, bone, lungs and tumor, the reconstructed model and the patient’s real body are registered. The four-dimensional heat map of the tumor as a reference to determine the space location of the puncture. In this paper, we conducted reconstructions of a four-dimensional heat map of region of interest and designed the best puncture path. The use of AR navigation technology to guide the puncture biopsy, which achieved the purpose of precise puncture, and facilitate the doctor to take a sample to do pathology analysis. Validations demonstrated our precise puncture system based on AR navigation and four-dimensional heat map reconstruction perform a greatly improved the accuracy of tumor puncture and the diagnoses rate of tumor biopsy. © 2018, Springer International Publishing AG.